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Fluorescent Ca2+ probe for high-resolution Ca2+ imaging

Research Project

Project/Area Number 25650055
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionWaseda University

Principal Investigator

SUZUKI Madoka  早稲田大学, 重点領域研究機構, 准教授 (40350475)

Co-Investigator(Kenkyū-buntansha) ARAI Satoshi  早稲田大学, 重点領域研究機構, 招聘研究員 (70454056)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords可視化 / 生物物理 / ナノ材料 / マイクロ・ナノデバイス / 細胞・組織
Outline of Final Research Achievements

The purpose of this study was to develop a novel fluorescent Ca2+ probe. In the first fiscal year (FY), we prepared new probes by using compounds newly synthesized. The microscopy setup was arranged to fit these probes. The probes were evaluated in HeLa cells under stimulus. However, in the second FY, we found that the fluorescence intensity was insufficient to obtain sufficient signal-to-noise ratio. Thus, we moved on to the next probe based on the knowledge gained in this study. We also incorporated the ability of targeting into the probe.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Microscopic heat pulse-induced calcium dynamics in single WI-38 fibroblasts2014

    • Author(s)
      Itoh, H., Oyama, K., Suzuki M., and Ishiwata, S.
    • Journal Title

      BIOPHYSICS

      Volume: 10 Issue: 0 Pages: 109-119

    • DOI

      10.2142/biophysics.10.109

    • NAID

      130004940803

    • ISSN
      1349-2942
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Single cell analysis of Ca2+ bursts in response to microscopic heat pulses.2015

    • Author(s)
      Itoh, H., Oyama, K., Suzuki, M., Lane, E.B., and Ishiwata, S.
    • Organizer
      UK-Singapore Skin Research Symposium
    • Place of Presentation
      Singapore
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Fast temperature measurement following single laser-induced cavitation inside a microfluidic gap.2014

    • Author(s)
      Madoka Suzuki, Pedro A. Quinto-Su and Claus-Deter Ohl
    • Organizer
      The 52st Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 筋肉Ca2+レベルの昆虫個体in vivo計測2014

    • Author(s)
      鈴木 団, Ferdinandus, 新井 敏,石渡信一,Hirotaka Sato
    • Organizer
      第20回藤目記念セミナー
    • Place of Presentation
      茨城
    • Year and Date
      2014-08-14 – 2014-08-15
    • Related Report
      2014 Annual Research Report
  • [Book] 熱パルスによる細胞機能の素早い変調. 「1分子生物学」(石渡信一、原田慶恵編)2014

    • Author(s)
      鈴木団
    • Publisher
      化学同人
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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