Fluorescent Ca2+ probe for high-resolution Ca2+ imaging
| Project/Area Number |
25650055
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Waseda University |
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Principal Investigator |
SUZUKI Madoka 早稲田大学, 重点領域研究機構, 准教授 (40350475)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Satoshi 早稲田大学, 重点領域研究機構, 招聘研究員 (70454056)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 可視化 / 生物物理 / ナノ材料 / マイクロ・ナノデバイス / 細胞・組織 |
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| Outline of Final Research Achievements |
The purpose of this study was to develop a novel fluorescent Ca2+ probe. In the first fiscal year (FY), we prepared new probes by using compounds newly synthesized. The microscopy setup was arranged to fit these probes. The probes were evaluated in HeLa cells under stimulus. However, in the second FY, we found that the fluorescence intensity was insufficient to obtain sufficient signal-to-noise ratio. Thus, we moved on to the next probe based on the knowledge gained in this study. We also incorporated the ability of targeting into the probe.
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Report
(3 results)
Research Products
(5 results)
