| Project/Area Number |
25650056
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
SUGASE Kenji 公益財団法人サントリー生命科学財団, その他部局等, 主席研究員 (00300822)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | in-cell NMR / タンパク質 / 揺らぎ / 高速測定 / 高速NMR法 |
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| Outline of Final Research Achievements |
We developed methods that enable rapid measurement of protein dynamics in cells using NMR. Specifically, we found that (i) a 2%-DMSO treatment of cells drastically enhanced the efficiency of protein incorporation into cells using the cell-penetrating peptide, (ii) presaturation of the water signal is useful for rapid NMR measurements to detect flexible regions in a protein, and (iii) the non-uniform sampling (NUS) method was utilized to reduce the total amount of experimental time and the SIFT algorithm, which we have been developing a computer program to reconstruct NUS data, was further developed. As a result, these newly developed methods enabled us to measure an NMR spectrum more rapidly by approximately one-third than the conventional measurement.
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