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Development of a high-resolution sample holder with lectin and binding protein on the thin film

Research Project

Project/Area Number 25650057
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Ogura Toshihiko  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 上級主任研究員 (70371028)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsバクテリア / ウイルス / 糖鎖 / 液中観察 / 走査電子顕微鏡 / レクチン / 細胞 / 窒化シリコン
Outline of Final Research Achievements

Cell infection of bacteria and virus is identified by the sugar chain and/or membrane proteins. Various sugar chains and biding proteins are located in the cell membrane, which is responsible for the important cell functions. Therefore, improving of its mechanism analysis will be helpful to various therapies. In this study, we developed a new observation method of bacteria and virus in water. This system allows for high-resolution imaging of various biological specimens with biding proteins and sugar chain under water condition. Our new method can be utilized for analysis of bacteria and virus with sugar chain and binding protein.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (1 results)

  • [Journal Article] 誘電率顕微鏡による液中の生物試料やナノ粒子の観察2016

    • Author(s)
      小椋 俊彦
    • Journal Title

      ぶんせき

      Volume: 496 Pages: 126-131

    • NAID

      40020825741

    • Related Report
      2015 Annual Research Report
  • [Journal Article] X-ray and cryo-EM structures reveal mutual conformational changes of Kinesin and GTP-state microtubules upon binding.2015

    • Author(s)
      Morikawa M, Yajima H, Nitta R, Inoue S, Ogura T, Sato C, Hirokawa N
    • Journal Title

      The EMBO Journal

      Volume: 34 Issue: 9 Pages: 1270-86

    • DOI

      10.15252/embj.201490588

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 走査電子顕微鏡による新規の液中観察技術の開発2015

    • Author(s)
      小椋 俊彦
    • Journal Title

      オレオサイエンス

      Volume: 15 Pages: 511-516

    • NAID

      130006329098

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM2015

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 459 Issue: 3 Pages: 521-528

    • DOI

      10.1016/j.bbrc.2015.02.140

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Non-destructive observation of intact bacteria and viruses in water by the highly sensitive frequency transmission electric-field method based on SEM2014

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 450 Issue: 4 Pages: 1684-1689

    • DOI

      10.1016/j.bbrc.2014.07.062

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Direct observation of unstained biological specimens in water by the frequency transmission electric-field method using SEM2014

    • Author(s)
      Toshihiko Ogura
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 3 Pages: e92780-e92780

    • DOI

      10.1371/journal.pone.0092780

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 変動電位透過観察法による水溶液中の非染色・非固定ウイルスの直接観察2014

    • Author(s)
      小椋 俊彦
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-11
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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