Elucidation of the molecular mechanism sensing lipid asymmetry and transducing the signals
| Project/Area Number |
25650059
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIHARA Akio 北海道大学, 薬学研究科(研究院), 教授 (50333620)
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| Co-Investigator(Renkei-kenkyūsha) |
OBARA Keisuke 北海道大学, 大学院薬学研究院, 助教 (30419858)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂質 / 脂質非対称 / スフィンゴ脂質 / グリセロリン脂質 / 細胞膜 / シグナル伝達 / Rim101経路 / 脂質二重層 / リン脂質 / アルカリ / ユビキチン化 / 生体膜 / フロッパーゼ |
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| Outline of Final Research Achievements |
Lipid composition differs between outer and inner leaflets of the plasma membrane, generating lipid asymmetry. Since lipid asymmetry is important for a variety of cellular functions, living organism contains a system maintaining lipid asymmetry. Previously, we found that Rim101 pathway responses to a change in lipid asymmetry and that Rim21 functions as a sensor. Here, we revealed that the signaling events from Rim21 to ESCRT III occured at the plasma membrane and ubiquitination was important. Furthermore, we identified a novel factor Opt2 as a protein induced by a change in lipid asymmetry in Rim21-dependent manner. We also revealed that Opt2 was essential for cell growth to respond to changes in lipid asymmetry.
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Report
(3 results)
Research Products
(9 results)
