A novel purification method for endogenous protein using ASKA technique

• Project/Area Number: 25650061
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: ICHIJO Hidenori 東京大学, 薬学研究科(研究院), 教授 (00242206)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ASK1 / 化合物プルダウン / シグナル複合体 / 複合体精製 / ケミカルバイオロジー / ASKAマウス

Outline of Final Research Achievements

ASK1 forms a huge complex with various molecules to sense wide variety of stresses and induce a diverse array of physiological responses. In this study, we established novel pull-down assay system for purifying ASK1 complex from a variety of tissues/cells, and we obtained dozens of novel components of ASK1 complex. Since ASK1 has different roles in different tissues/cells, we are going to elucidate how ASK1 modulates diverse kinds of stress responses in each tissue/cell.

Research Products

• [Journal Article] Mitochondrial proteolysis: Its emerging roles in stress responses. (2015)
  • Author(s): Sekine, S. and Ichijo, H.
  • Journal Title: B.B.A. Volume: 1850 Issue: 2 Pages: 274-280
  • DOI: 10.1016/j.bbagen.2014.10.012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The DEAH-Box RNA Helicase DHX15 activates NF-κB and MAPK signaling downstream of MAVS during antiviral responses (2014)
  • Author(s): Mosallanejad, K., Sekine, Y., Ishikura-Kinoshita, S., Kumagai, K., Nagano, T., Matsuzawa, A., Takeda, K., Naguro, I. and Ichijo, H.
  • Journal Title: Sci. Signal. Volume: 7 Issue: 323
  • DOI: 10.1126/scisignal.2004841
  • Peer Reviewed
• [Journal Article] Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to regulate stress responses (2014)
  • Author(s): Maruyama, T., Araki, T., Kawarazaki, Y., Naguro, I., Heynen, S., Aza-Blanc, P., Ronai, Z., Matsuzawa, A. and Ichijo, H.
  • Journal Title: Sci. Signal. Volume: 7 Issue: 309
  • DOI: 10.1126/scisignal.2004822
  • Peer Reviewed / Open Access
• [Journal Article] Lysosome rupture-activated TAK1-JNK pathway regulates NLRP3 inflammasome activation. (2014)
  • Author(s): Okada, M., Matsuzawa, A., Yoshimura, A. and Ichijo, H.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 47 Pages: 32926-32936
  • DOI: 10.1074/jbc.m114.579961
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ASK1 promotes the contact hypersensitivity response through IL-17 production (2014)
  • Author(s): Mizukami, J. Sato, T., Camps, M., Ji, H., Rueckle, T., Swinnen, D., Tsuboi, R., Takeda, K. and Ichijo, H
  • Journal Title: Sci. Rep. Volume: 4 Issue: 1 Pages: 4714-4714
  • DOI: 10.1038/srep04714
  • NAID: 120006986607
  • Peer Reviewed / Open Access
• [Journal Article] Depletion of apoptosis signal-regulating kinase 1 prevents bile duct ligation-induced necroinflammation and subsequent peribiliary fibrosis. (2014)
  • Author(s): Noguchi, H., Yamada, S., Nabeshima, A., Guo, X., Tanimoto, A., Wang, K.Y., Kitada, S., Tasaki, T., Takama, T., Shimajiri, S., Horlad, H., Komohara, Y., Izumi, H., Kohno, K., Ichijo, H. and Sasaguri, Y.
  • Journal Title: Am. J. Pathol. Volume: 184 Issue: 3 Pages: 644-661
  • DOI: 10.1016/j.ajpath.2013.11.030
  • Peer Reviewed
• [Journal Article] The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts. (2014)
  • Author(s): Matsui, H., Fukuno, N., Kanda, Y., Kantoh, Y., Chida, T., Nagaura, Y., Suzuki, O., Nishitoh, H., Takeda, K., Ichijo, H., Sawada, Y., Sasaki, K., Kobayashi, T. and Tamura, S.
  • Journal Title: J. Biol. Chem. Volume: 289 Issue: 10 Pages: 6438-6450
  • DOI: 10.1074/jbc.m113.536300
  • Peer Reviewed / Open Access
• [Journal Article] Apoptosis signal-regulating kinase 1 is a novel target molecule for cognitive impairment induced by chronic cerebral hypoperfusion. (2014)
  • Author(s): Toyama K, Koibuchi N, Uekawa K, Hasegawa Y, Kataoka K, Katayama T, Sueta D, Ma MJ, Nakagawa T, Yasuda O, Tomimoto H, Ichijo H, Ogawa H, Kim-Mitsuyama S
  • Journal Title: Arteriosclerosis, thrombosis, and vascular biology Volume: 34 Issue: 3 Pages: 616-625
  • DOI: 10.1161/atvbaha.113.302440
  • Peer Reviewed
• [Journal Article] Requirement of apoptosis-inducing kinase 1 for the induction of bronchial asthma following stimulation with ovalbumin. (2013)
  • Author(s): Takada, E., Furuhata, M., Nakae, S., Ichijo, H., Sudo, K. and Mizuguchi, J.
  • Journal Title: Int. Arch. Allergy Immunol. Volume: 162 Issue: 2 Pages: 104-114
  • DOI: 10.1159/000353240
  • Peer Reviewed
• [Journal Article] SOD1 as a molecular switch for initiating the homeostatic ER stress respouse under zinc deficiency. (2013)
  • Author(s): Homma, K., Fujisawa. T., Tsuburaya, N., Yamaguchi, N., Kadowaki, H., Takeda, K., Nishitoh, H., Matsuzawa, A., Naguro, I., and Ichijo, H.
  • Journal Title: Molecular Cell Volume: 52 Issue: 1 Pages: 75-86
  • DOI: 10.1016/j.molcel.2013.08.038
  • Peer Reviewed
• [Journal Article] Integration of the Apoptosis signal-regulating kinase 1-mediated stress signaling with the Akt/PKB-IκB kinase cascade (2013)
  • Author(s): Puckett, M.C., Goldman, E.H., Cockrell, L.M., Huang, B., Kasinski, A.L., Du, Y., Wang, C.Y., Lin, A., Ichijo, H., Khuri, F. and Fu, H.
  • Journal Title: Mol. Cell. Biol. Volume: 33 Issue: 11 Pages: 2252-2259
  • DOI: 10.1128/mcb.00047-13
  • Peer Reviewed
• [Journal Article] Involvement of ASK1-p38 pathway in the pathogenesis of diabetes triggered by pancreatic s cell exhaustion (2013)
  • Author(s): Yamaguchi, K., Takeda, K., Kadowaki, H., Ueda, I., Namba, Y., Ouchi, Y. Nishitoh, H. and Ichijo, H.
  • Journal Title: Biochim. Biophys. Acta. Volume: 1830 Issue: 6 Pages: 3656-3663
  • DOI: 10.1016/j.bbagen.2013.01.029
  • Peer Reviewed
• [Presentation] ミトコンドリアストレスシグナルの機能解析 (2014)
  • Author(s): 一條秀憲
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] SOD1 as a molecular switch for initiating the homeostatic ER stress response under conditions of zinc deficiency (2014)
  • Author(s): Homma, K., Ichijo, H.
  • Organizer: The 4th International ISZB Meeting
  • Place of Presentation: CA, USA
  • Year and Date: 2014-09-14 – 2014-09-19
• [Presentation] Oxidative stress-activated kinase ASK1 is involved in the tumorigenesis and tumor metastasis (2014)
  • Author(s): Naguro, I., Kamiyama, M., Ichijo, H.
  • Organizer: Thiol-Based Redox Regulation & Signaling(Gordon Research Conference)
  • Place of Presentation: Girona, Spain
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] Ubiquitin-dependent regulation of ASK1 stress signaling in cell death (2014)
  • Author(s): Hidenori Ichijo
  • Organizer: 2014 KSBMB Annual Meeting
  • Place of Presentation: COEX, Seaul, Korea
  • Year and Date: 2014-05-14 – 2014-05-16
• [Presentation] Regulation of cell death signals by ubiquitination and deubiquitination (2013)
  • Author(s): Hidenori Ichijo
  • Organizer: The 35th Naito Conference The Ubiquitin-proteasome system-
  • Place of Presentation: 北海道札幌市
• [Presentation] レドックス場としてのASK1シグナル複合体と細胞死 (2013)
  • Author(s): 一條秀憲
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Stress Signaling in Cell Death and Disease (2013)
  • Author(s): Hidenori Ichijo
  • Organizer: The 8th Japan-Korea conference on cellular signalling for young scientists
  • Place of Presentation: 福岡県福岡市