The cooperative effect of actin barbed end regulators on controlling the filament turnover mechanisms

• Project/Area Number: 25650064
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: Takeda Shuichi 名古屋大学, 理学(系)研究科(研究院), 研究員 (50509081)
• Co-Investigator(Renkei-kenkyūsha): NARITA Akihiro 名古屋大学, 大学院理学研究科, 准教授 (30360613)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アクチン / アクチンダイナミックス / アクチンキャッピングタンパク質 / X線結晶構造解析 / 細胞運動

Outline of Final Research Achievements

Cell motility is driven by a dynamic assembly of a cytoskeletal protein, actin that exists in two states; monomeric form (called G-actin) or filamentous form (actin filaments). The actin capping protein (CP) binds tightly to the end of actin filaments and stops the assembly and disassembly of the filaments, thus playing an important role in controlling the cellular dynamics. In this study, by using X-ray crystallography, we have revealed the modes of interaction between CP and its binding partners. Our results suggest that, by regulating the filament end-binding affinity of CP, cells control the amount of the monomeric form actin to achieve collective migration.

Research Products

• [Journal Article] Comprehensive analysis of motions in molecular dynamics trajectories of the actin capping protein and its inhibitor complexes (2016)
  • Author(s): Koike, R., Takeda, S., Maeda, Y., Ota, M.
  • Journal Title: Proteins Volume: 未定 Issue: 7 Pages: 948-956
  • DOI: 10.1002/prot.25043
  • Peer Reviewed
• [Journal Article] Effect of lipid composition and solution condition on the mechanical property of membrane vesicle (2015)
  • Author(s): Kato, N., Ishijima, A., Inaba, T., Nomura, F., Takeda, S., Takiguchi, K.
  • Journal Title: Membranes Volume: 5 Pages: 22-47
  • Peer Reviewed / Open Access
• [Presentation] Crystal structure of FA2 demonstrates filamentous actin structure (2016)
  • Author(s): Takeda, S., Maeda, Y.
  • Organizer: Alpbach meeting; Myosin, muscle and many motors
  • Place of Presentation: Alpbach, Austria
  • Year and Date: 2016-03-12
  • Int'l Joint Research
• [Presentation] 動的構造に着目したCARMIL蛋白質によるキャップ蛋白質の機能阻害メカニズムの解明 (2015)
  • Author(s): 小池 亮太郎、武田 修一、前田 雄一郎、太田 元規
  • Organizer: 第15回日本蛋白質科学会年会
  • Place of Presentation: あわぎんホール、徳島市
  • Year and Date: 2015-06-24
• [Presentation] アクチンのMgポリマーについて (2015)
  • Author(s): 菊本真人、武田修一、前田雄一郎
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 学習院大学、札幌
  • Year and Date: 2015-01-07 – 2015-01-09
• [Presentation] “Mg-Polymer”再考 (2014)
  • Author(s): Kikumoto, M., Takeda, S., Matsumoto, T., Maeda, Y.
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌
  • Year and Date: 2014-09-13 – 2014-09-15
• [Presentation] How the binding of CP (capping protein) to the B-end of actin filament is regulated (2014)
  • Author(s): Takeda, S., Koike, R., Ota, M., Maeda, Y.
  • Organizer: European Muscle Conference
  • Place of Presentation: Salzburg, Austria
  • Year and Date: 2014-09-10 – 2014-09-14
• [Presentation] How the binding of CP (capping protein) to the B-end of actin filament is regulated (2014)
  • Author(s): Takeda, S., Koike, R., Ota, M., Maeda, Y.
  • Organizer: Gordon Resaerch Conference, Muscle and Motor Proteins
  • Place of Presentation: Mount Snow, West Dover, VT, USA
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] How an intrinsically disordered region functions: a case of CARMIL protein (2014)
  • Author(s): Ota, M., Takeda, S., Maeda, Y., Koike, R.
  • Organizer: The 4th APPA（Asia Pacific Protein Association）Conference
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] CARMIL蛋白質の天然変性領域によるアロステリックなキャップ蛋白質の機能制御 (2013)
  • Author(s): 小池 亮太郎、武田 修一、前田 雄一郎、太田 元規
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館（鳥取市）
  • Invited