Generation of an eukaryote harboring genes transferred from mitochondria to the nucleus
| Project/Area Number |
25650067
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
OKAMOTO Koji 大阪大学, 生命機能研究科, 准教授 (40455217)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | ミトコンドリア / ミトコンドリアDNA / 酵母 / 合成生物学 / オミックス |
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| Outline of Final Research Achievements |
Mitochondria possess their own genome whose oxidative stress-induced mutations are thought to cause intractable disorders such as mitochondrial disease and aging. In this study, we aimed to transfer genes from mitochondria to the nucleus and express them. Particularly, we sought to (1) change codon usage for nuclear genes, (2) optimize codon usage for efficient protein synthesis via cytosolic ribosomes, and (3) target synthesized polypeptides to mitochondria by adding a presequence.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling2015
Author(s)
Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K
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Journal Title
EMBO Journal
Volume: 34
Issue: 21
Pages: 2703-2719
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.2015
Author(s)
Murakawa T, Yamaguchi O, Hashimoto A, Hikoso S, Takeda T, Oka T, Yasui H, Ueda H, Akazawa Y, Nakayama H, Taneike M, Misaka T, Omiya S, Shah AM, Yamamoto A, Nishida K, Ohsumi Y, Okamoto K, Sakata Y, Otsu K.
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Journal Title
Nat Commun.
Volume: 6
Issue: 1
Pages: 7527-7527
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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