Generation of an eukaryote harboring genes transferred from mitochondria to the nucleus

• Project/Area Number: 25650067
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: OKAMOTO Koji 大阪大学, 生命機能研究科, 准教授 (40455217)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ミトコンドリア / ミトコンドリアDNA / 酵母 / 合成生物学 / オミックス

Outline of Final Research Achievements

Mitochondria possess their own genome whose oxidative stress-induced mutations are thought to cause intractable disorders such as mitochondrial disease and aging. In this study, we aimed to transfer genes from mitochondria to the nucleus and express them. Particularly, we sought to (1) change codon usage for nuclear genes, (2) optimize codon usage for efficient protein synthesis via cytosolic ribosomes, and (3) target synthesized polypeptides to mitochondria by adding a presequence.

Research Products

• [Journal Article] Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling (2015)
  • Author(s): Sakakibara K, Eiyama A, Suzuki SW, Sakoh-Nakatogawa M, Okumura N, Tani M, Hashimoto A, Nagumo S, Kondo-Okamoto N, Kondo-Kakuta C, Asai E, Kirisako H, Nakatogawa H, Kuge O, Takao T, Ohsumi Y, Okamoto K
  • Journal Title: EMBO Journal Volume: 34 Issue: 21 Pages: 2703-2719
  • DOI: 10.15252/embj.201591440
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Protein N-terminal Acetylation by the NatA Complex Is Critical for Selective Mitochondrial Degradation (2015)
  • Author(s): Eiyama A, Okamoto K
  • Journal Title: Journal of Biological Chemistry Volume: 290 Issue: 41 Pages: 25034-25044
  • DOI: 10.1074/jbc.m115.677468
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. (2015)
  • Author(s): Murakawa T, Yamaguchi O, Hashimoto A, Hikoso S, Takeda T, Oka T, Yasui H, Ueda H, Akazawa Y, Nakayama H, Taneike M, Misaka T, Omiya S, Shah AM, Yamamoto A, Nishida K, Ohsumi Y, Okamoto K, Sakata Y, Otsu K.
  • Journal Title: Nat Commun. Volume: 6 Issue: 1 Pages: 7527-7527
  • DOI: 10.1038/ncomms8527
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PINK1/Parkin-mediated mitophagy in mammalian cells (2015)
  • Author(s): Eiyama A, Okamoto K
  • Journal Title: Current Opinion in Cell Biology Volume: 33 Pages: 95-101
  • DOI: 10.1016/j.ceb.2015.01.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Assays for autophagy II: Mitochondrial autophagy. (2014)
  • Author(s): Kanki T, Okamoto K.
  • Journal Title: Methods Mol Biol. Volume: 1163 Pages: 165-173
  • DOI: 10.1007/978-1-4939-0799-1_11
  • ISBN: 9781493907984, 9781493907991
  • Peer Reviewed
• [Journal Article] Receptor-mediated mitophagy in yeast and mammalian systems (2014)
  • Author(s): Liu L, Sakakibara K, Chen Q, Okamoto K.
  • Journal Title: Cell Research Volume: 24 Issue: 7 Pages: 787-795
  • DOI: 10.1038/cr.2014.75
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial degradation during starvation is selective and temporally distinct from bulk autophagy in yeast. (2013)
  • Author(s): Eiyama, A., Kondo-Okamoto, N., and Okamoto, K.
  • Journal Title: FEBS Lett. Volume: 587 Issue: 12 Pages: 1787-1792
  • DOI: 10.1016/j.febslet.2013.04.030
  • Peer Reviewed
• [Presentation] Targeting autophagy for mitochondrial clearance (2015)
  • Author(s): Okamoto K
  • Organizer: 2015 TSMRM Symposium on Mitochondrial Dysfunction in Human Diseases and Annual Meeting
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2015-12-05
  • Invited
• [Presentation] Multiple pathways regulating mitochondria-specific autophagy (2015)
  • Author(s): Okamoto K
  • Organizer: Cold Spring Harbor Asia Conference on Mitochondria
  • Place of Presentation: Suzhou, China
  • Year and Date: 2015-10-12
  • Int'l Joint Research / Invited
• [Presentation] マイトファジー：ミトコンドリアを丸ごと分解する分子機構と生理機能 (2015)
  • Author(s): 岡本浩二
  • Organizer: 第34回分子病理学研究会
  • Place of Presentation: ホテル フルーツ・フラワー、神戸
  • Year and Date: 2015-07-25
  • Invited
• [Presentation] オートファジーが駆動する選択的ミトコンドリア分解 (2015)
  • Author(s): 岡本浩二
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学（兵庫県）
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] Targeting autophagy for mitochondrial clearance (2015)
  • Author(s): Okamoto K
  • Organizer: 7th International Symposium on Autophagy
  • Place of Presentation: Huangshan (China)
  • Year and Date: 2015-03-21
  • Invited
• [Presentation] ミトコンドリアを丸ごと隔離・除去する仕組み (2014)
  • Author(s): 岡本浩二
  • Organizer: 第25回フォーラム・イン・ドージン
  • Place of Presentation: 熊本ホテルキャッスル（熊本県）
  • Year and Date: 2014-11-14
  • Invited
• [Presentation] リン脂質メチル化を介した選択的ミトコンドリア分解の制御 (2014)
  • Author(s): 岡本浩二
  • Organizer: 第9回臨床ストレス応答学会大会
  • Place of Presentation: 岡山大学（岡山県）
  • Year and Date: 2014-11-01
  • Invited
• [Presentation] 酵母が語る選択的ミトコンドリア分解の基本原理 (2014)
  • Author(s): 岡本浩二
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都府）
  • Year and Date: 2014-10-18
  • Invited
• [Presentation] An unexpected link between phospholipid methylation and mitophagy (2014)
  • Author(s): Okamoto K
  • Organizer: 2nd International Picobiology Institute Symposium
  • Place of Presentation: 兵庫県立大学（兵庫県）
  • Year and Date: 2014-10-10
  • Invited
• [Presentation] オートファジーが駆動するミトコンドリア分解の仕組み (2014)
  • Author(s): 岡本浩二
  • Organizer: 第52回日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] Targeting autophagy for mitochondrial clearance (2014)
  • Author(s): Okamoto K
  • Organizer: 4th Asia Pacific Protein Association Conference
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2014-05-18
  • Invited
• [Presentation] Targeting autophagy for mitochondrial clearance (2014)
  • Author(s): Koji Okamoto
  • Organizer: Keystone Symposium on MItochondrial Dynamics and Physiology
  • Place of Presentation: Santa Fe, New Mexico, USA
  • Invited
• [Presentation] リン脂質代謝の異常から垣間見えてきたマイトファジーの分子機構 (2013)
  • Author(s): 岡本 浩二
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜、横浜
  • Invited
• [Presentation] Targeting autophagy for mitochondrial clearance (2013)
  • Author(s): Koji Okamoto
  • Organizer: 10th Conference of the Asian Society of Mitochondrial Research and Medicine (ASMRM)
  • Place of Presentation: Seoul, South Korea
  • Invited
• [Remarks] 研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/okamoto/Okamoto_Lab/
• [Remarks] 研究内容紹介サイト
  • URL: http://www.omoroi-seimei.info/introduction/lab27-okamoto.html
• [Remarks] 研究室ホームページ
  • URL: http://www.fbs.osaka-u.ac.jp/labs/okamoto/Okamoto_Lab/
• [Remarks] 研究内容紹介サイト
  • URL: http://www.omoroi-seimei.info/introduction/lab27-okamoto.html