Picobiological analysis of the ER and Golgi stress responses

• Project/Area Number: 25650070
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: University of Hyogo
• Principal Investigator: YOSHIDA Hiderou 兵庫県立大学, 生命理学研究科, 教授 (60378528)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 小胞体 / ゴルジ体 / ストレス応答 / 構造生物学 / 結晶構造解析 / 転写 / スプライシング / シャペロン / SPring-8 / Ｘ線自由電子レーザー / 細胞生物学

Outline of Final Research Achievements

Secretory proteins are synthesized and folded with endoplasmic reticulum (ER) chaperones in the ER, and received various post-translational modifications such as glycosylation. The ER and Golgi stress responses are the mechanisms that augment function of each organelle in accordance with cellular demands. In this project, we planned to reveal the mammalian molecular mechanisms of the ER and Golgi stress responses through the structural analyses of pATF6(P), pXBP1(U) and TFE3, key regulators of the ER and Golgi stress response. Finaly, we obtained pXBP1(U) protein for the analysis of X-ray crystallography.

Research Products

• [Journal Article] TFE3 Is a bHLH-ZIP-type Transcription Factor that Regulates the Mammalian Golgi Stress Response (2015)
  • Author(s): Taniguchi, M., Nadanaka, S., Tanakura, S., Sawaguchi, S., Midori, S., Kawai, Y., Yamaguchi, S., Shimada, Y., Nakamura, Y., Matsumura, Y., Fujita, N., Araki, N., Yamamoto, M., Oku, M., Wakabayashi, S., Kitagawa, H., Yoshida, H.
  • Journal Title: Cell Structure and Function Volume: 40 Issue: 1 Pages: 13-30
  • DOI: 10.1247/csf.14015
  • NAID: 130004703720
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Endoplasmic reticulum stress in kidney function and disease. (2015)
  • Author(s): Taniguchi, M. and Yoshida, H.
  • Journal Title: Current Opinion in Nephrology and Hypertension Volume: in press
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Organelle autoregulation-stress responses in the ER, Golgi, mitochondria and lysosome. (2015)
  • Author(s): Sasaki, K. and Yoshida, H.
  • Journal Title: J Biochem. Volume: 157 Pages: 185-195
  • NAID: 40020428705
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] UBC9 Regulates the Stability of XBP1, a Key Transcription Factor Controlling the ER Stress Response (2013)
  • Author(s): Aya Uemura, Yusaku Matsuo, Masaya Oku, Mai Taniguchi, Sadao Wakabayashi and Hiderou Yoshida
  • Journal Title: Cell Structure and Function Volume: 38 Issue: 1 Pages: 67-79
  • DOI: 10.1247/csf.12026
  • NAID: 130004053893
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum stress and apoptosis contribute to the pathogenesis of dominantly inherited isolated GH deficiency due to GH1 gene splice site mutations. (2013)
  • Author(s): Ariyasu D, Yoshida H, Yamada M, Hasegawa Y.
  • Journal Title: Endocrinology Volume: 154 Issue: 9 Pages: 3228-3239
  • DOI: 10.1210/en.2013-1249
  • Peer Reviewed
• [Journal Article] The essential biology of the endoplasmic reticulum stress response for structural and computational biologists. (2013)
  • Author(s): Wakabayashi S, Yoshida H.
  • Journal Title: Comput Struct Biotechnol J Volume: e201303010 Issue: 7 Pages: 1-9
  • DOI: 10.5936/csbj.201303010
  • Peer Reviewed
• [Presentation] ゴルジ体ストレス応答による糖鎖修飾の制御 (2014)
  • Author(s): Hiderou Yoshida
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-26
  • Invited
• [Presentation] The novel response pathway of the mammalian Golgi stress response that regulates glycosylation of proteoglycans in the Golgi apparatus (2014)
  • Author(s): Hiderou Yoshida
  • Organizer: EMBO Conference
  • Place of Presentation: バルセロナ（スペイン）
  • Year and Date: 2014-10-28 – 2014-10-29
• [Presentation] A novel Golgi stress response pathway that regulates proteoglycan glycosylation (2014)
  • Author(s): Hiderou Yoshida
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都国際会館（京都府京都市）
  • Year and Date: 2014-10-17
  • Invited
• [Presentation] Mammalian Golgi stress response regulating Golgi glycosylation. (2014)
  • Author(s): Hiderou Yoshida
  • Organizer: JSCB presymposium
  • Place of Presentation: 東大寺文化センター（奈良県奈良市）
  • Year and Date: 2014-06-10
  • Invited
• [Presentation] ER Stress Response and Golgi Stress Response (2014)
  • Author(s): Hiderou Yoshida
  • Organizer: Protein and Peptide Conference 2014
  • Place of Presentation: 大連（中国）
  • Year and Date: 2014-04-26
  • Invited
• [Presentation] ER stress response and Golgi stress response (2014)
  • Author(s): Yoshida H.
  • Organizer: ISN Forefront Symposium
  • Place of Presentation: Charleston（アメリカ合衆国）
  • Invited
• [Presentation] ゴルジ体ストレス応答における膜タンパク質の品質管理 (2013)
  • Author(s): 吉田秀郎
  • Organizer: 日本分子生物学会 第36回年会
  • Place of Presentation: 神戸国際展示場（兵庫県）
  • Invited
• [Presentation] 細胞分化とゴルジ体ストレス応答 (2013)
  • Author(s): 吉田秀郎
  • Organizer: 日本生化学会 第86回大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
  • Invited
• [Presentation] ゴルジ体ストレス応答の分子的実体：不完全な糖鎖修飾の関与 (2013)
  • Author(s): 吉田秀郎
  • Organizer: 日本細胞生物学会 第65回大会
  • Place of Presentation: ウインク愛知（愛知県）
• [Remarks] Hiderou Yoshida, University of Hyogo
  • URL: http://www.sci.u-hyogo.ac.jp/life/biochem2/index-j.html
• [Remarks] 兵庫県立大学大学院生命理学研究科 吉田秀郎研究室
  • URL: https://sites.google.com/site/hiderouoshidalab2/home
• [Remarks] Hiderou Yoshida Lab
  • URL: https://sites.google.com/site/hiderouyoshidalab/home?