Identification of the enzyme gene involved in the BMP biosynthesis

• Project/Area Number: 25650072
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MOTO Ken-ichi 独立行政法人理化学研究所, 小林脂質生物学研究室, 専任研究員 (90333335)
• Co-Investigator(Renkei-kenkyūsha): SAKAI Shota 北海道大学, 先端生命科学研究院, 特任助教 (60611720)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: BMP / ホスホリパーゼ / 後期エンドソーム / LPLAT / リパーゼ

Outline of Final Research Achievements

Bis(monoacylglycero)phosphate (BMP) is a unique phospholipid enriched in the late endosome. There are some questionable points about the function of BMP and the de novo biosynthesis of BMP has not been clearly demonstrated. In this study, I tried to identify a novel enzyme gene involved in the BMP biosynthesis and establish a cell line that the amount of BMP is decreased by silencing of the gene.
From the result of proteome analysis of endosome, I found some acyltransferases and some phospholipases which may be involved in the BMP biosynthesis. Gene silencing indicated that one of the phospholipases was involved in the BMP biosynthesis. Finally I succeeded to establish the cell line the amount of BMP is decreased by silencing of the gene.

Research Products

• [Remarks] 独立行政法人理化学研究所 小林脂質生物学研究室
  • URL: http://www.riken.jp/lbl/mainpagelbl.html