Development of the experimental system in which nuclear envelope can be assembled around a biomolecule-coated bead in a living cell
| Project/Area Number |
25650073
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Information and Communications Technology |
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Principal Investigator |
KOBAYASHI Shouhei 独立行政法人情報通信研究機構, 未来ICT研究所バイオICT研究室, 主任研究員 (40425765)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 核膜 / DNAビーズ / BAF / autophagy / live CLEM / DNAセンサー / オートファジー回避 / 人工細胞核 / ビーズ |
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| Outline of Final Research Achievements |
This research aims to develop an experimental system in which nuclear envelope (NE) can be artificially assembled around a biomolecule-coated bead in a living cell. Live cell imaging-associated correlative light and electron microscopy of the beads coated with candidate molecules which are known to act as a seed of NE formation in the cell extract-based NE reconstitution experiments revealed that DNA-bound and BAF-bound beads can assemble nuclear envelope-like membranes around the beads in living cells. Further studies about the NE-like membranes formed around the DNA-coated beads revealed that BAF-mediated assembly of the NE-like membranes leads to exogenous DNA avoiding autophagy. Based on these results we conclude that our bead-mediated system can be used to induce and dissect the process of NE assembly in living cells.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy.2015
Author(s)
Kobayashi,S., Koujin, T., Kojidani, T., Osakada, H., Mori, C., Hiraoka, Y., Haraguchi, T.
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America
Volume: 印刷中
Issue: 22
Pages: 7027-7032
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Externally Controllable Molecular Communication Systems.2014
Author(s)
Nakano, T., Kobayashi, S., Suda, T., Okaie, Y., Hiraoka, Y., Haraguchi, T.
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Journal Title
IEEE Journal of Selected Areas in Communications
Volume: 32
Pages: 2417-2431
DOI
Related Report
Peer Reviewed / Open Access
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