Research Project
Grant-in-Aid for Challenging Exploratory Research
This research aims to develop an experimental system in which nuclear envelope (NE) can be artificially assembled around a biomolecule-coated bead in a living cell. Live cell imaging-associated correlative light and electron microscopy of the beads coated with candidate molecules which are known to act as a seed of NE formation in the cell extract-based NE reconstitution experiments revealed that DNA-bound and BAF-bound beads can assemble nuclear envelope-like membranes around the beads in living cells. Further studies about the NE-like membranes formed around the DNA-coated beads revealed that BAF-mediated assembly of the NE-like membranes leads to exogenous DNA avoiding autophagy. Based on these results we conclude that our bead-mediated system can be used to induce and dissect the process of NE assembly in living cells.
All 2015 2014 2013 Other
All Journal Article (5 results) (of which Peer Reviewed: 5 results, Open Access: 3 results, Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 1 results) Book (1 results) Remarks (1 results)
Proceedings of the National Academy of Sciences of the United States of America
Volume: 印刷中 Issue: 22 Pages: 7027-7032
10.1073/pnas.1501235112
IEEE Journal of Selected Areas in Communications
Volume: 32 Pages: 2417-2431
10.1145/2619955.2619971
J. Cell Biol.
Volume: 203 Issue: 1 Pages: 115-128
10.1083/jcb.201304188
Journal of the NICT
Volume: 60 Pages: 41-44
130008037059
NICT季報
Volume: 59 Pages: 37-40
http://www.nict.go.jp/press/2015/05/19-1.html