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identification of regulatory factors for generation of neurons by using live imaging of embryos

Research Project

Project/Area Number 25650076
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

SEHARA Atsuko  京都大学, 再生医科学研究所, 教授 (60209038)

Project Period (FY) 2013-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords細胞分化 / 増殖因子 / 神経分化 / ゼブラフィッシュ / ライブイメージング / ニューレグリン
Research Abstract

During brain development, neural progenitor cells (NPCs) proliferate and produce post-mitotic neurons. The extent of NPC expansion and the timing of neuron production should impact on the size and shape of the brain. Here, we show that neuronal generation from NPCs is not a stochastic event, but instead a spatially and temporally regulated process and depends on Neuregulin 1 type II (NRG1-II), an isoform of an EGF-related ligand NRG1, using the optic tectum (OT) of zebrafish embryos as a model system. Knocking-down NRG1-II or treatment with AG1478, an ErbB inhibitor, impaired neuron production from sub-basal NPCs prominently. The removal of AG1478 resumed sub-basal mitoses without affecting mitoses in the apical ventricular (V) region. Injection of soluble human NRG1 into the brain ameliorated neurogenesis of NRG1-II-depleted embryos, implying involvements of NRG1-ErbB signaling in neuron production in the developing vertebrate brain.

Report

(2 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (14 results)

All 2014 2013 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (11 results) (of which Invited: 11 results)

  • [Journal Article] Neureglin-1/Glial Growth Factor stimulates Schwann Cell Migration by including the α5β1 integrin-ErbB2-Focal Adhesion Kinase Complex Formation.2014

    • Author(s)
      Wakatsuki, S., Araki, T., Sehara-Fujisawa, A..
    • Journal Title

      Genes to Cells

      Volume: 19(1) Issue: 1 Pages: 66-77

    • DOI

      10.1111/gtc.12108

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fetal Skeletal Muscle Progenitors Have Regenerative Capacity After Intramuscular Engraftment in Dystrophin Deficient Mice.2013

    • Author(s)
      Sakai, H., et al
    • Journal Title

      PLoS ONE

      Volume: 8(5) Issue: 5 Pages: e63016-e63016

    • DOI

      10.1371/journal.pone.0063016

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.2013

    • Author(s)
      Tanaka, A., et al
    • Journal Title

      PLoS ONE

      Volume: 8(4) Issue: 4 Pages: e61540-e61540

    • DOI

      10.1371/journal.pone.0061540

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Roles of ADAM19 in cranial nerve development2014

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      The 6th Asia Oceania Zebrafish Conference
    • Place of Presentation
      中国、香港
    • Year and Date
      2014-01-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Roles of ADAM Proteases in Development of Zebrafish2013

    • Author(s)
      Fuminori Sato, Hiroyuki Kangawa, Hiroyuki Arai, Kazuya Tsumagari, Atsuo Kawahara, Koichi Kawakami, Atsuko Sehara-Fujisawa
    • Organizer
      the 8th General Meeting of the International Proteolysis Society
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2013-10-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 生命誕生の設計図を解く2013

    • Author(s)
      瀬原淳子
    • Organizer
      第6回形態科学シンポジウム「医学・生物学研究の魅力を語る:高校生のための集い」
    • Place of Presentation
      京都府京都市
    • Year and Date
      2013-10-12
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Exploring Mechanisms of Cellular Differentiation by 3-and 4-dimensional (3D+time) imaging2013

    • Author(s)
      Atsuko Sehara
    • Organizer
      Kickoff Meeting of Imaging Platform for Spatio-Temporal Information
    • Place of Presentation
      Kyoto University
    • Year and Date
      2013-05-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Exploring Roles of ADAM proteases Using Zebrafish2013

    • Author(s)
      Atsuko Sehara
    • Organizer
      Swiss-Kyoto Symposium
    • Place of Presentation
      Zurich, Swiss
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Invited
  • [Presentation] Exploring Roles of ADAM proteases Using Zebrafish2013

    • Author(s)
      Atsuko Sehara
    • Organizer
      京都大学シンポジウム
    • Place of Presentation
      国立台湾大学
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Exploring Roles of ADAM proteases Using Zebrafish2013

    • Author(s)
      Atsuko Sehara
    • Organizer
      国立台湾大学―京都大学シンポジウム
    • Place of Presentation
      台湾、台北
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Exploring Mechanisms of Cellular Differentiation by 3- and 4-dimensional (3D+time) imaging.

    • Author(s)
      Atsuko Sehara
    • Organizer
      Kickoff Meeting of Imaging Platform for Spatio-Temporal Information, KyotoUniversity
    • Place of Presentation
      京都府京都市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 生命誕生の設計図を解く

    • Author(s)
      瀬原淳子
    • Organizer
      第6回形態科学シンポジウム「医学・生物学研究の魅力を語る:高校生のための集い」
    • Place of Presentation
      京都府京都市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Roles of ADAM Proteases in Development of Zebrafish.

    • Author(s)
      Fuminori Sato, et al.
    • Organizer
      the 8th General Meeting of the International Proteolysis Society
    • Place of Presentation
      Cape Town, South Africa
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Roles of ADAM19 in cranial nerve development.

    • Author(s)
      Atsuko Sehara-Fujisawa
    • Organizer
      The 6th Asia Oceania Zebrafish Conference
    • Place of Presentation
      中国、香港
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2014-07-25   Modified: 2016-11-25  

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