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An attempt to identify genes involved in PGC specification by using haploid ES cells

Research Project

Project/Area Number 25650077
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyushu University (2014)
Kyoto University (2013)

Principal Investigator

HAYASHI Katsuhiko  九州大学, 医学(系)研究科(研究院), 教授 (20287486)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords始原生殖細胞 / ES細胞 / 遺伝子スクリーニング / 一倍体 / 順遺伝学的スクリーニング
Outline of Final Research Achievements

Primordial germ cells (PGCs) are origin of all germ cell lineage including sperm and egg. During embryogenesis PGCs are specified at an early developmental stage. This study aims to identify genes involved in PGC specification by using haploid embryonic stem cell lines, which are convenient for forward genetics to identify functional genes. Thus far, we have constructed a plasmid vector that interfere endogenous gene expression at the integrated genomic locus. By transfection of the plasmid vector to haploid ES cells, we have made a pool of ES cell lines in which multiple copies of the vectors are inserted in the genome. Based on the established materials, we are going to screen functional genes involved in PGC specification.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 8 results) Book (3 results)

  • [Journal Article] Hayashi K, Saitou M.2014

    • Author(s)
      Perspectives of germ cell development in vitro in mammals.
    • Journal Title

      Animal Science Journal

      Volume: 85(6) Issue: 6 Pages: 617-626

    • DOI

      10.1111/asj.12199

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Generation of eggs from mouse embryonic stem cells and induced plurip otent stem cells2013

    • Author(s)
      Hayashi K, Saitou M
    • Journal Title

      Nature Protocols

      Volume: 8 Issue: 8 Pages: 1513-1524

    • DOI

      10.1038/nprot.2013.090

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Induction of mouse germ-cell fate by transcription factors in vitro.2013

    • Author(s)
      Nakaki F, Hayashi K, Ohta H, Kurimoto K, Yabuta Y, Saitou M.
    • Journal Title

      Nature

      Volume: 501 Issue: 7466 Pages: 222-226

    • DOI

      10.1038/nature12417

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stepwise differentiation from naive state pluripotent stem cells to functional primordial germ cells through an epiblast-like state.2013

    • Author(s)
      Hayashi K and Saitou M
    • Journal Title

      Methods in molecular biology

      Volume: 1074 Pages: 175-183

    • DOI

      10.1007/978-1-62703-628-3_13

    • ISBN
      9781627036276, 9781627036283
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tsix RNA and the germline factor, PRDM14, link X-reactivation and stem cell reprogramming2013

    • Author(s)
      Payer B, Rosenberg M, Yamaji M, Yabuta Y, Michiyo K-A, Hayashi K. Yamanaka S. Saitou M, Lee J. T.
    • Journal Title

      Molecular Cell

      Volume: 52 Issue: 6 Pages: 805-818

    • DOI

      10.1016/j.molcel.2013.10.023

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants2013

    • Author(s)
      Aramaki, S., Hayashi, K., Kurimoto, K., Ohta, H., Yabuta, Y., Iwanari, H., Mochizuki, Y., Hamakubo, T., Kato, Y., Shirahige, K., and Saitou, M
    • Journal Title

      Developmental Cell

      Volume: 27 Issue: 5 Pages: 516-529

    • DOI

      10.1016/j.devcel.2013.11.001

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Propagating generations of healthy mice using manufactured oocytes2015

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      Ovarian Club V
    • Place of Presentation
      香港
    • Year and Date
      2015-01-31 – 2015-02-02
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Generation of eggs from mouse embryonic stem cells2014

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      Society of Reproduction and Fertility
    • Place of Presentation
      イギリス
    • Year and Date
      2014-09-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] In vitro germline formation from pluripotent stem cells2014

    • Author(s)
      林克彦
    • Organizer
      第47回日本発生生物学会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Prospects of gamete production from pluripotent stem cells2014

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      The 45th meeting of the Dutch Society of Obstetrics and Gynaecology
    • Place of Presentation
      オランダ
    • Year and Date
      2014-05-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Making Oocytes and Babies from Stem Cells2014

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      The Congress of the Asia Pacific Initiative on Reproduction
    • Place of Presentation
      オーストラリア
    • Year and Date
      2014-04-04 – 2014-04-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 生殖細胞系列の体外培養による再構築2013

    • Author(s)
      林克彦
    • Organizer
      第25回高遠・分子細胞生物学シンポジウム
    • Place of Presentation
      大津市 延暦寺会館
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Germ cell differentiation from stem cells in mice2013

    • Author(s)
      Katsuhiko Hayashi
    • Organizer
      17th World COngress on IVF
    • Place of Presentation
      Tunis, Tunisia
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 始原生殖細胞の分化を体外で再現する培養系の確立と利用2013

    • Author(s)
      林克彦
    • Organizer
      第85回日本遺伝学会
    • Place of Presentation
      横浜市 慶應義塾大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 生殖細胞系列の再構築培養系の確立と生殖巣の役割2013

    • Author(s)
      林克彦
    • Organizer
      第21回日本ステロイドホルモン学会
    • Place of Presentation
      大阪市 千里ライフセンター
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 実験医学2013

    • Author(s)
      林克彦、斎藤通紀
    • Publisher
      羊土社
    • Related Report
      2013 Research-status Report
  • [Book] HORMONE FRONTIER IN GYNECOLOGY2013

    • Author(s)
      林克彦、斎藤通紀
    • Publisher
      メディカルレビュー社
    • Related Report
      2013 Research-status Report
  • [Book] 産科と婦人科2013

    • Author(s)
      林克彦、斎藤通紀
    • Publisher
      診断と治療社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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