Research Project
Grant-in-Aid for Challenging Exploratory Research
Primordial germ cells (PGCs) are origin of all germ cell lineage including sperm and egg. During embryogenesis PGCs are specified at an early developmental stage. This study aims to identify genes involved in PGC specification by using haploid embryonic stem cell lines, which are convenient for forward genetics to identify functional genes. Thus far, we have constructed a plasmid vector that interfere endogenous gene expression at the integrated genomic locus. By transfection of the plasmid vector to haploid ES cells, we have made a pool of ES cell lines in which multiple copies of the vectors are inserted in the genome. Based on the established materials, we are going to screen functional genes involved in PGC specification.
All 2015 2014 2013
All Journal Article (6 results) (of which Peer Reviewed: 6 results, Open Access: 2 results, Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 8 results) Book (3 results)
Animal Science Journal
Volume: 85(6) Issue: 6 Pages: 617-626
10.1111/asj.12199
Nature Protocols
Volume: 8 Issue: 8 Pages: 1513-1524
10.1038/nprot.2013.090
Nature
Volume: 501 Issue: 7466 Pages: 222-226
10.1038/nature12417
Methods in molecular biology
Volume: 1074 Pages: 175-183
10.1007/978-1-62703-628-3_13
Molecular Cell
Volume: 52 Issue: 6 Pages: 805-818
10.1016/j.molcel.2013.10.023
Developmental Cell
Volume: 27 Issue: 5 Pages: 516-529
10.1016/j.devcel.2013.11.001