An attempt to identify genes involved in PGC specification by using haploid ES cells
Project/Area Number |
25650077
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
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Research Institution | Kyushu University (2014) Kyoto University (2013) |
Principal Investigator |
HAYASHI Katsuhiko 九州大学, 医学(系)研究科(研究院), 教授 (20287486)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 始原生殖細胞 / ES細胞 / 遺伝子スクリーニング / 一倍体 / 順遺伝学的スクリーニング |
Outline of Final Research Achievements |
Primordial germ cells (PGCs) are origin of all germ cell lineage including sperm and egg. During embryogenesis PGCs are specified at an early developmental stage. This study aims to identify genes involved in PGC specification by using haploid embryonic stem cell lines, which are convenient for forward genetics to identify functional genes. Thus far, we have constructed a plasmid vector that interfere endogenous gene expression at the integrated genomic locus. By transfection of the plasmid vector to haploid ES cells, we have made a pool of ES cell lines in which multiple copies of the vectors are inserted in the genome. Based on the established materials, we are going to screen functional genes involved in PGC specification.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants2013
Author(s)
Aramaki, S., Hayashi, K., Kurimoto, K., Ohta, H., Yabuta, Y., Iwanari, H., Mochizuki, Y., Hamakubo, T., Kato, Y., Shirahige, K., and Saitou, M
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Journal Title
Developmental Cell
Volume: 27
Issue: 5
Pages: 516-529
DOI
Related Report
Peer Reviewed / Open Access
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