The role of Lefty1 expression cells during the ES cell self-organization.

Research Project

Project/Area Number	25650079
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Developmental biology
Research Institution	Osaka University
Principal Investigator	TAKAOKA Katsuyoshi 大阪大学, 生命機能研究科, 助教 (90551044)
Project Period (FY)	2013-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords	再生医療 / 自己組織化 / ES細胞 / 人工臓器 / 位置情報 / 未分化 / 非対称性の起源
Research Abstract	The acquirement of positional informations is essential for the making of structurally-complicated artificial organs. In mouse embryos, Lefty1 is a key player during the Left-right and anterior-posterior axis formation. And, it is known that Lefty1 is expressed is ES (Embryonic Stem) cells. In this study, I have tried to reveal the role of Lefty1 expression cells in the self-organization of ES cells. As a result, time-lapse observations of Lefty1 expressing cells in the formation of colony showed that the expression of Lefty1 started in edge cells of the ES colony. And, Lefty1,2 null ES cells normally formed colonies. Hereafter, I'm going to reveal which do Lefty1,2 null ES cells form embryoid bodies.

Report

(2 results)