| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
It remains unclear how the gene networks regulating development in multicellular organisms evolved. The experimental variation of such evolutionary processes has been possible only in limited cases. In the present study, we aimed to establish a method for in vivo reconstruction of gene networks using genome-editing technologies. We used a model insect, Gryllus bimaculatus, and achieved targeted gene knockout and knockin with CRISPR/Cas9 system. In addition, we produced genetic strains expressing a marker gene in a similar pattern of a targeted gene via enhancer trap. Thus, we established a technical basis for experimental modification of gene networks by modifying cis-regulatory sequences.
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