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Nuclear-mitochondrial interactions in somatic cell senescence in budding tunicates

Research Project

Project/Area Number 25650081
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKochi University

Principal Investigator

KAWAMURA Kaz  高知大学, 自然科学系, 教授 (30136361)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsホヤ / 出芽 / 加齢 / ミトコンドリア / TFAM / Prohibitin 2 / ヒストンメチル化 / ヒストンアセチル化 / ミトコンドリア転写因子a / プロヒビティン2 / チトクロームcオキシダーゼ1 / Prohibitin2 / 呼吸鎖遺伝子群 / クロマチン免疫沈降
Outline of Final Research Achievements

In the budding tunicate, Polyandrocarpa misakiensis, mitochondrial respiratory genes are repeatedly activated and inactivated in relation to budding and zooidal senescence. In this research project, it was examined how and to what extent 2 nuclear genes, mitochondrial transcription factor a (PmTFAM) and prohibitin 2 (PmPHN2), were involved in gene activation of mitochondrial cytochrome c oxidase subunit 1 (PmCOX1). Both PmTFAM and PmPHN2 were most abundantly expressed during budding stages and attenuated during ageing. An endogenous humoral factor, TC14-3, regulated PmTFAM and PmPHN2 gene expression via histone methylation and PmPHN2 was also influenced by histone acetylation. RNAi of PmTFAM caused PmCOX1 downregulation, and the transfection of PmTFAM mRNA upregulated PmCOX1 expression. These results strongly suggest that PmTFAM and PmPHN2 mediate the nuclear control of mitochondrial genes through histone modification.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (3 results)

  • [Journal Article] Histone mechylation codes involved in stemness, multipotency, and senescence in budding tunicates.2015

    • Author(s)
      Kawamura K, Kinoshita M, Sekida S, Sunanaga T.
    • Journal Title

      Mechanisms of Ageing and Development

      Volume: 145 Pages: 1-12

    • DOI

      10.1016/j.mad.2014.12.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Interactive histone acetylation and methylation in regulating transdifferentiation-related genes during tunicate budding and regeneration.2015

    • Author(s)
      Shibuya M, Hatano M, Kawamura K.
    • Journal Title

      Developmental Dynamics

      Volume: 244 Issue: 1 Pages: 10-20

    • DOI

      10.1002/dvdy.24212

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Senescence-associated superoxide dismutase influences mitochondrial gene expression in budding tunicates.2013

    • Author(s)
      Kawamura, K. and Sunanaga, T.
    • Journal Title

      Development Growth & Differentiation

      Volume: 55 Issue: 5 Pages: 606-614

    • DOI

      10.1111/dgd.12065

    • NAID

      40019735147

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Retinoid X receptor-mediated transdifferentiation cascade in budding tunicates.2013

    • Author(s)
      Kawamura, K., Shiohara, M., Kanda, M. and Fujiwara, S.
    • Journal Title

      Dev. Biol.

      Volume: 384 Issue: 2 Pages: 343-355

    • DOI

      10.1016/j.ydbio.2013.10.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ホヤの出芽・再生・加齢におけるヒストンコード2014

    • Author(s)
      川村和夫・砂長毅
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学川内北キャンパス 宮城県仙台市
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Screening and identification of differentially expressed genes in gonadal tissues in colonial ascidian, Botryllus primigenus.2013

    • Author(s)
      Sunanaga, T., Kuroda, S., Otsuki, M. and Kawamura, K.
    • Organizer
      国際ホヤミーティング
    • Place of Presentation
      イタリア、ナポリ
    • Related Report
      2013 Research-status Report
  • [Presentation] 群体ホヤの生殖腺形成に関わる遺伝子群の単離2013

    • Author(s)
      砂長毅・大月恵・黒田紗希・川村和夫
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県岡山市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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