| Project/Area Number |
25650082
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
OHTA KUNIMASA 熊本大学, 大学院生命科学研究部, 准教授 (90244128)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 乳酸菌 / 細胞 / リプログラム / 多能性 / リプログラミング |
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| Outline of Final Research Achievements |
Previously, we demonstrated the generation of multipotential cells from adult human dermal fibroblast (HDF) cells by incorporating lactic acid bacteria (LAB) (Ohta et al., PLOS ONE e51866, 2012). LAB-incorporated cell clusters differentiated into endodermal, mesodermal, and ectodermal cells in vivo and in vitro.
Next, we purified the LAB-derived materials from the homogenized LAB through ion-changed and gel-filtration chromatography by observing their cell clusters forming activity. We applied the peak fraction to MALDI-TOF-MS analysis and determined the LAB-derived reprogramming material. When we applied the LAB-derived material to HDF cells, they formed cell clusters and differentiated into cells derived from three germ layers. LAB-derived material was incorporated into the cells by endocytosis and they localized not only in the cytoplasmic region but also in the nucleus．Thus, LAB-derived material reprograms the host cells and modify their gene expression patterns.
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