Analysis of flp-10 that controls organogenesis in c.elegans

• Project/Area Number: 25650085
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kwansei Gakuin University
• Principal Investigator: KIM Hon-Song 関西学院大学, 理工学研究科, 博士研究員 (70469899)
• Research Collaborator: MORITA Keisuke
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 器官形成 / 細胞移動 / 神経ペプチド / 線虫 / ペプチド

Outline of Final Research Achievements

An FMRFamide has Phe-Met-Arg-Phe-NH2 in its carboxyl terminus. These peptides are known to be involved in regulation of neuronal activities. We found that the C.elegans FLP-10C peptide, a part of the proteolytic fragment of FMRFamide like peptide (FLP), controls tip cell migration of U-shaped gonads in C.elegans. This suggests a novel function of this peptide in regulating gonadogenesis. We identified a protein in the worm lysate specifically co-precipitated with the FLP-10C peptide in a pull-down assay using the biotinylated peptide. LC/MS/MS analysis revealed that the specific protein corresponded to the product of H28O16.1. H28O16.1 encodes the alpha subunit of mitochondrial ATP synthase. Interestingly, there are some reports that the mitochondrial ATP synthase is also expressed in the plasma membrane. These results suggest that H28O16.1 could be the receptor for the FLP-10C peptide to regulate gonadogenesis.

Research Products

• [Journal Article] The novel secreted factor MIG-18 acts with MIG-17/ADAMTS to control cell migration in Caenorhabditis elegans. (2014)
  • Author(s): Kim H-S, Kitano Y, Mori M, Takano T, Harbaugh TE, Mizutani K, Yanagimoto H, Miwa S, Ihara S, Kubota Y, Shibata Y, Ikenishi K, Garriga G and Nishiwaki K.
  • Journal Title: Genetics Volume: 196(2) Issue: 2 Pages: 471-479
  • DOI: 10.1534/genetics.113.157685
  • URL: http://www.genetics.org/content/196/2/471.long
  • Peer Reviewed / Open Access
• [Presentation] 線虫の生殖巣リーダー細胞の移動を制御するflp-10遺伝子の解析 (2014)
  • Author(s): 森田啓介、仲矢圭吾、金憲誠、西脇清二
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Analysis of flp-10 that controls migration of the gonadal leader cells in C.elegans (2014)
  • Author(s): 森田啓介、金憲誠、西脇清二
  • Organizer: C.elegans Development, Cell Biology and Gene Expression Meeting in association with The 6th Asia-Pacific C.elegans meeting
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2014-07-15 – 2014-07-19
• [Presentation] ペプチドが制御する新たな器官形成機構について (2013)
  • Author(s): 金憲誠
  • Organizer: 日本発生生物学会2013年秋期シンポジウム
  • Place of Presentation: 神戸市、しあわせの村
• [Presentation] Nuclear membrane proteins act in transport of the Netrin receptor, UNC-5 in cell migration in C. elegans.
  • Author(s): Hon-Song Kim and Kiyoji Nishiwaki
  • Organizer: 第46回発生生物学会年会
  • Place of Presentation: 島根県松江、くにびきメッセ
• [Presentation] Nuclear membrane proteins act in transport of the Netrin receptor, UNC-5 in cell migration in C. elegans.
  • Author(s): Hon-Song Kim and Kiyoji Nishiwaki
  • Organizer: 19th International C.elegans meeting
  • Place of Presentation: UCLA, Los Angels, USA