Establishsment of the efficient gene knock-in technique in the Drosophila genome
Project/Area Number |
25650088
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Developmental biology
|
Research Institution | Kumamoto University |
Principal Investigator |
NAKAMURA Akira 熊本大学, 発生医学研究所, 教授 (90323245)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | ショウジョウバエ / TALEN / CRISPR/Cas9 / 遺伝子ノックアウト / ゲノム編集 / 相同組み換え / CRISPR/Cas / 相同組換え |
Outline of Final Research Achievements |
To accelerate artificial manipulations of the Drosophila genome in the post-genome project era, we sough to establish genome editing methodologies in Drosophila using TALEN and CRISPR/Cas9 technologies. We have succeeded in establishing protocols for gene knockout as well as knock-in through homologous recombination. Although gene knock-in efficiencies were varied from a few percent to ~90%, depending on targets, we have already generated GFP knocked-in strains in seven different loci, indicating that the gene knock-in methodology in Drosophila has been practically established.
|
Report
(3 results)
Research Products
(7 results)
-
[Journal Article] Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression2015
Author(s)
Kim, G., Pai, C.-I., Sato, K., Person, M. D., Nakamura, A. and Macdonald, P. M.
-
Journal Title
PLoS Genetics
Volume: 11
Issue: 2
Pages: e1004992-e1004992
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Endophilin B is required for the Drosophila oocyte to endocytose yolk downstream of Oskar2014
Author(s)
1. Tsai, Y. C., Chang, W., Liou, W., Lee, W. H., Chang, Y. W., Wang, P. Y., Tanaka, T., Nakamura, A. and Pai, L. M.
-
Journal Title
Development
Volume: 141
Issue: 3
Pages: 563-573
DOI
Related Report
Peer Reviewed
-
-
-
-
-