| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
To accelerate artificial manipulations of the Drosophila genome in the post-genome project era, we sough to establish genome editing methodologies in Drosophila using TALEN and CRISPR/Cas9 technologies. We have succeeded in establishing protocols for gene knockout as well as knock-in through homologous recombination. Although gene knock-in efficiencies were varied from a few percent to ~90%, depending on targets, we have already generated GFP knocked-in strains in seven different loci, indicating that the gene knock-in methodology in Drosophila has been practically established.
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