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Molecular mechanism of cell-cell communication via plasmodesmata

Research Project

Project/Area Number 25650089
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionHokkaido University

Principal Investigator

Fujita Tomomichi  北海道大学, 理学(系)研究科(研究院), 准教授 (50322631)

Co-Investigator(Renkei-kenkyūsha) Motomura Taizo  北海道大学, 北方生物圏フィールド科学センター, 教授 (30183974)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsplasmodesmata / physcomitrella patens / abscisic acid / ヒメツリガネゴケ / 光変換蛍光タンパク質 / 細胞間コミュニケーション / 原形質連絡 / アブシジン酸 / 活性酸素 / 光変換蛍光タンンパク質 / ストレス応答 / ABI1 / ABI3
Outline of Final Research Achievements

Cell-cell communication via plasmodesmata is important to control growth and environmental response in plants. However, the molecular mechanism is largely unknown.
We developed a quantitative method to analyze cell-cell communication by using a photoconvertible fluorescence protein, Dendra2 in protonemata of the moss, Physcomitrella patens, and studied a regulation of Dendra2 movement. We found two factors that are involved in abscisic acid signaling play a key role to control abscisic acid-dependent suppression of Dendra2 movement.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] A model system for analyzing intercellular communication through plasmodesmata using moss protonemata and leaves.2015

    • Author(s)
      Kitagawa, Munenori and Fujita, Tomomichi
    • Journal Title

      Journal of Plant Research

      Volume: 128 Issue: 1 Pages: 63-72

    • DOI

      10.1007/s10265-014-0690-7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Plasmodesmata: function and diversity in plant intercellular communication.2015

    • Author(s)
      Fujita, Tomomichi
    • Journal Title

      Journal of Plant Research

      Volume: 128 Issue: 1 Pages: 3-5

    • DOI

      10.1007/s10265-014-0697-0

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Quantitative imaging of directional transport through plasmodesmata in moss protonemata viasingle-cell photoconversion of Dendra22013

    • Author(s)
      Kitagawa, M. and Fujita, T
    • Journal Title

      Journal of Plant Research

      Volume: 126 Issue: 4 Pages: 577-585

    • DOI

      10.1007/s10265-013-0547-5

    • NAID

      10031184112

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] アブシジン酸シグナル伝達経路を 介した原形質連絡の制御2016

    • Author(s)
      友井拓実,北川宗典,坂田洋一, 藤田知道
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県・盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] アブシジン酸シグナル伝達経路を介した原形質連絡の制御2015

    • Author(s)
      友井拓実、北川宗典、坂田洋一、藤田知道
    • Organizer
      日本植物学会
    • Place of Presentation
      朱鷺メッセ(新潟県・新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒメツリガネゴケがもつ4つのAN3ホモログの発現様式2015

    • Author(s)
      川出健介、藤田知道
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular analysis of signaling related to abscisic acid-dependent plasmodesmatal regulation2015

    • Author(s)
      Takumi Tomoi, Munenori Kitagawa, Yoichi Sakata, Tomomichi Fujita
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] Abscisic Acid-dependent Regulation of Plasmodesmata Function2015

    • Author(s)
      Munenori Kitagawa, Takumi Tomoi, Yoichi Sakata, Mayuko Sato, Kiminori Toyooka, Hitoshi Sakakibara1, Tomomichi Fujita
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Research-status Report
  • [Presentation] アブシジン酸依存的な原形質連絡制御におけるシグナル分子の解析2014

    • Author(s)
      北川宗典、友井拓実、坂田洋一、豊岡公徳、佐藤繭子、榊原均、藤田知道
    • Organizer
      日本植物学会第78会大会
    • Place of Presentation
      明治大学(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Regulation of cell-cell diffusion of macromolecules via plasmodesmata in filamentous tissue of the moss Physcomitrella patens2014

    • Author(s)
      Kitagawa, Munenori, and Fujita, Tomomichi
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山大学、富山市
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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