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Establishment of a novel Poaceae model sytem: a case study of environmental stress response

Research Project

Project/Area Number 25650090
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionIwate University

Principal Investigator

UEMURA MATSUO  岩手大学, 農学部, 教授 (00213398)

Research Collaborator NAKAYAMA Takato  
TAKAHASHI Daisuke  
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords環境応答 / モデル植物 / ミナトカモジグサ / Brachypodium / 細胞膜 / 低温馴化 / 乾燥ストレス / 塩ストレス
Outline of Final Research Achievements

To develop a novel mode plant experimental system, stress responses of Brachypodium distachyon was evaluated. The results obtained revealed that 1) freezing tolerance increased from -4C in non-acclimated plants to -7.5C in 1-week-cold acclimated plants, 2) CBF transcription factors and their target genes, CORs and LEAs, were upregulated during cold acclimation, 3) the proteomes of the plasma membrane that is the primary site of freezing injury changed considerably in response to cold, and 4) the plant can be tolerated for the treatment of 100 mM NaCl for 2 weeks or even after stopping of water for 10 days. These results clearly show that B. distachyon is promising as a monocotyledonous model plant experimental system for research of environmental stress responses.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results) (of which Invited: 2 results) Book (4 results)

  • [Journal Article] 植物の低温馴化および凍結耐性メカニズムに関する基礎研究.2014

    • Author(s)
      上村松生
    • Journal Title

      低温生物工学会誌

      Volume: 60 Pages: 1-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant plasma membrane proteomics for improving cold tolerance.2013

    • Author(s)
      Takahashi Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 4 Pages: 90-90

    • DOI

      10.3389/fpls.2013.00090

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 低温馴化過程におけるB. distachyon細胞膜タンパクの組成変動の解析2013

    • Author(s)
      中山貴人、高橋大輔、河村幸男、Abidur Rahman、上村松生
    • Journal Title

      低温生物工学会誌

      Volume: 59 Pages: 61-65

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Plasma membrane proteome dynamically and strategically responds to cold acclimation in plants.2014

    • Author(s)
      Uemura M, Takahashi D, Nakayama T, Miki Y, Kawamura Y.
    • Organizer
      6th International Symposium on Frontiers in Agricultural Proteome Research
    • Place of Presentation
      Heilongjiang University, Harbin, PRC
    • Year and Date
      2014-06-23 – 2014-06-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 新規単子葉モデル植物・ミナトカモジグサにおける低温馴化機構2014

    • Author(s)
      中山貴人,高橋大輔,河村幸男,上村松生
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山大学、富山
    • Related Report
      2013 Research-status Report
  • [Presentation] Plasma membrane proteomics in association with cold acclimation2013

    • Author(s)
      Nakayama T, Kawamura Y, Uemura M.
    • Organizer
      1st International Brachypodium Conference
    • Place of Presentation
      Universty of Modena, Modena, Italy
    • Related Report
      2013 Research-status Report
  • [Presentation] Cold-acclimation-induced changes of plasma membrane proteome in Brachypodium distachyon2013

    • Author(s)
      Nakayama T, Takahashi D, Kawamura Y, Uemura M
    • Organizer
      12th International Wheat Genetics Symposium
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Involvement of the plasma membrane in plant cold adaptation: a proteomics perspective2013

    • Author(s)
      Takahashi D, Nakayama T, Miki Y, Kawamura Y, Uemura M
    • Organizer
      12th Human Proteome Organisation Congress
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] 単子葉モデル植物ミナトカモジグサを用いた低温馴化機構の解析2013

    • Author(s)
      中山貴人,高橋大輔,河村幸男,上村松生
    • Organizer
      東北植物学会第3回大会
    • Place of Presentation
      カレッジプラザ、秋田
    • Related Report
      2013 Research-status Report
  • [Book] Proteomic approaches to identify cold regulated plasma membrane proteins. In: Methods in Molecular Biology, vol 1066, Plant Cold Acclimation: Methods and Protocols (Hincha DK, Zuther E, eds)2014

    • Author(s)
      Takahashi D, Nakayama T, Miki Y, Kawamura Y, Uemura M.
    • Total Pages
      282
    • Publisher
      Springer Science+Business Media, LLC,
    • Related Report
      2014 Annual Research Report
  • [Book] Food production and supply issues in northern Japan in the era of global climate change and the role of the United Graduate School of Agricultural Sciences, Iwate University. In: Sustainable Agriculture and Food Security in the Circumpolar North (Seefeldt S, Helfferich D, eds)2014

    • Author(s)
      Uemura M.
    • Total Pages
      132
    • Publisher
      Alaska Agricultural & Forestry Experiment Station
    • Related Report
      2014 Annual Research Report
  • [Book] Proteomic approaches to identify cold regulated plasma membrane proteins. (Plant Cold Acclimation: Methods and Protocols, Hincha DK, Zuther E, eds)2014

    • Author(s)
      Takahashi D, Nakayama T, Miki Y, Kawamura Y, Uemura M
    • Publisher
      Springer Science+Business Media, LL
    • Related Report
      2013 Research-status Report
  • [Book] Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS. Methods in Molecular Biology (Plant Proteomics: Methods and Protocols, 2nd Ed, Novo JVJ, Komatsu S, Weckwerth W, Wienkoopeds S, eds)2013

    • Author(s)
      Takahashi D, Li B, Nakayama T, Kawamura Y, Uemura M.
    • Total Pages
      786
    • Publisher
      Springer Science+Business Media, LLC
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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