Establishment of a novel Poaceae model sytem: a case study of environmental stress response
Project/Area Number |
25650090
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Iwate University |
Principal Investigator |
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Research Collaborator |
NAKAYAMA Takato
TAKAHASHI Daisuke
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 環境応答 / モデル植物 / ミナトカモジグサ / Brachypodium / 細胞膜 / 低温馴化 / 乾燥ストレス / 塩ストレス |
Outline of Final Research Achievements |
To develop a novel mode plant experimental system, stress responses of Brachypodium distachyon was evaluated. The results obtained revealed that 1) freezing tolerance increased from -4C in non-acclimated plants to -7.5C in 1-week-cold acclimated plants, 2) CBF transcription factors and their target genes, CORs and LEAs, were upregulated during cold acclimation, 3) the proteomes of the plasma membrane that is the primary site of freezing injury changed considerably in response to cold, and 4) the plant can be tolerated for the treatment of 100 mM NaCl for 2 weeks or even after stopping of water for 10 days. These results clearly show that B. distachyon is promising as a monocotyledonous model plant experimental system for research of environmental stress responses.
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Report
(3 results)
Research Products
(13 results)
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[Book] Proteomic approaches to identify cold regulated plasma membrane proteins. In: Methods in Molecular Biology, vol 1066, Plant Cold Acclimation: Methods and Protocols (Hincha DK, Zuther E, eds)2014
Author(s)
Takahashi D, Nakayama T, Miki Y, Kawamura Y, Uemura M.
Total Pages
282
Publisher
Springer Science+Business Media, LLC,
Related Report
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[Book] Shotgun proteomics of plant plasma membrane and microdomain proteins using nano-LC-MS/MS. Methods in Molecular Biology (Plant Proteomics: Methods and Protocols, 2nd Ed, Novo JVJ, Komatsu S, Weckwerth W, Wienkoopeds S, eds)2013
Author(s)
Takahashi D, Li B, Nakayama T, Kawamura Y, Uemura M.
Total Pages
786
Publisher
Springer Science+Business Media, LLC
Related Report