Establishment of standard cell line in fern plant for the analysis of cell proliferation
| Project/Area Number |
25650091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 培養細胞 / シダ植物 / プロトプラスト |
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| Outline of Final Research Achievements |
Focusing on the fern plant to make the suspension culture for establishing the standard cell line suitable for analysis of the proliferation. The spores of Ceratopteris richardii were aseptically germinated and transplanted to the gellan gum medium and agar medium of various hormone levels to induce callus. From some good-growing calluses, the suspension cultures were established. The cell lines were stably maintained in a LS liquid medium. Further, the protoplasts were isolated using Cellulase RS and Pectolyase Y23, and then were subsequently cultured. This is a rare study result in fern plants.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-22013
Author(s)
Kariya, K., Demiral, T., Sasaki, T., Tsuchiya, Y., Turkan, I., Sano, T., Hasezawa, S., Yamamoto, Y.
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Journal Title
J. Inorg. Biochem.
Volume: 128
Pages: 196-201
DOI
Related Report
Peer Reviewed
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