Molecular properties of signal transducing proteins on the plasma membrane

• Project/Area Number: 25650093
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: MAESHIMA Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞内情報変換 / 細胞膜 / カルシウム / ホスファチジルイノシトール / 情報伝達 / 情報変換 / イノシトールリン脂質 / シグナル伝達 / ホスファチジルイノシトールリン酸

Outline of Final Research Achievements

We investigated molecular properties of the plasma membrane Ca2+-binding proteins, PCaP1 and PCaP2, at biochemical and molecular biological levels. We found that PCaP2 is essential for formation and tip growth of root hairs using loss-of-function mutant lines of PCaP2. Our key finding is that PCaP2 transduces a Ca2+ signal to phosphatidylinositol signal on the plasma membrane at tip of root hair. When the N-terminal peptide of PCaP2 was introduced to plants under the control of CaM 35S promoter, no root hair was observed in the transformant. Using this mutant, we quantified physiological functions of root hair, such as absorption of water and mineral ions. Also we obtained structural information of PCaP1 on the binding to membrane phospholipids and metal ions and found that loss-of-function mutant of PCaP1 has a defect in stomatal closure in the dark.

Research Products

• [Journal Article] The histidine-rich loop of Arabidopsis vacuolar membrane zinc transporter AtMTP1 is involved in sensing cytosolic level of zinc. (2015)
  • Author(s): Tanaka, N., Fujiwara, T., Tomioka, R., Krämer, U., Kawachi, M., and Maeshima, M.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 3 Pages: 510-519
  • DOI: 10.1093/pcp/pcu194
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 6 Pages: 1497-1512
  • DOI: 10.1093/jxb/eru014
  • Peer Reviewed / Open Access
• [Journal Article] Dynamics of vacuoles and H+-pyrophosphatase visualized by monomeric green fluorescent protein in Arabidopsis: Artifactual bulbs and native intravacuolar spherical structures. (2014)
  • Author(s): Segami, S., Makino, S., Miyake, A., Asaoka, M., and Maeshima, M.
  • Journal Title: Plant Cell Volume: 26 Issue: 8 Pages: 3416-3434
  • DOI: 10.1105/tpc.114.127571
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the critical residues for the function of vacuolar H+-pyrophosphatase by amino acid screening based on the 3D structure. (2014)
  • Author(s): Asaoka, M., Segami, S., and Maeshima, M.
  • Journal Title: Journal of Biochemistry Volume: 156 Issue: 6 Pages: 333-344
  • DOI: 10.1093/jb/mvu046
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata. (2013)
  • Author(s): Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U., and Lee, Y.
  • Journal Title: Plant Cell Volume: 25 Issue: 6 Pages: 2202-2216
  • DOI: 10.1105/tpc.113.110411
  • Peer Reviewed
• [Journal Article] The Ca2+-binding protein PCaP2 located on the plasma membrane is involved in root hair development as a possible signal transducer (2013)
  • Author(s): Kato, M., et. al.
  • Journal Title: Plant Journal Volume: (印刷中) Issue: 4 Pages: 690-700
  • DOI: 10.1111/tpj.12155
  • Peer Reviewed
• [Presentation] 根毛伸長時におけるシロイヌナズナCa2+結合タンパク質PCaP2とホスファチジルイノシトール-4,5二リン酸の細胞内動態. (2014)
  • Author(s): 加藤真理子、前島正義、青山卓史
  • Organizer: 植物脂質科学研究会シンポジウム
  • Place of Presentation: 静岡市産学交流センター
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] 新規シグナル変換分子PCaP1は植物の水分屈性に関与している. (2014)
  • Author(s): 田中奈月、高橋秀幸、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] リン酸欠乏時に発現上昇する根毛タンパク質RHPPの機能解析. (2014)
  • Author(s): 田中奈月、奥田祥平、Ferjani Ali、郡司玄、前島正義
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Organizer: Plant Biology Europe FESPB/EPSO 2014 Congress
  • Place of Presentation: Doublin, Irland
  • Year and Date: 2014-06-21 – 2014-06-27
• [Presentation] 細胞膜局在型新規Ca2+結合タンパク質PCaP1 の構造とリガンド結合の解析 (2013)
  • Author(s): 相羽孝亮、長崎-武内菜穂子、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: パシフィコ横浜