Study on plant systemic signaling via phloem
| Project/Area Number |
25650095
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 移動性mRNA / 篩管 / 長距離移行 / 長距離シグナル伝達 / 接木 / 高速シークエンサー |
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| Outline of Final Research Achievements |
Phloem is a conductive tissue that allocates nutrients from mature source leaves to sinks such as young developing tissues. Phloem also delivers proteins and RNA species, such as small RNAs and mRNAs. Intensive studies on plant systemic signaling revealed the essential roles for proteins and RNA species. However, many of their functions are still largely unknown. A major difficulty is the absence of an accurate and comprehensive list of mobile transcripts. In this study, we used a hetero-graft system with Nicotiana benthamiana as the recipient scion and Arabidopsis as the donor stock, to identify transcripts that moved long distances across the graft union. We identified 138 Arabidopsis transcripts as mobile mRNAs, which we collectively termed the mRNA mobilome. RT-PCR, qRT-PCR and droplet digital PCR analyses confirmed the mobility. The transcripts included potential signaling factors and, unexpectedly, more general factors.
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Report
(3 results)
Research Products
(15 results)
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[Presentation] タバコ属を用いた異科接木の挑戦2015
Author(s)
野田口理孝, 佐藤良勝, 東山哲也
Organizer
園芸学会 平成27年度春季大会
Place of Presentation
千葉県千葉市, 千葉大学
Year and Date
2015-03-28 – 2015-03-29
Related Report
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[Presentation] タバコ属を用いた異科接木の挑戦2015
Author(s)
野田口理孝, 佐藤良勝, 東山哲也
Organizer
日本育種学会 第127回講演会
Place of Presentation
東京都町田市, 玉川大学
Year and Date
2015-03-21 – 2015-03-22
Related Report
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[Presentation] タバコ属を用いた異科接木への挑戦2015
Author(s)
野田口理孝, 佐藤良勝, 東山哲也
Organizer
日本植物生理学会 第56回年会
Place of Presentation
東京都世田谷区, 東京農業大学
Year and Date
2015-03-16 – 2015-03-18
Related Report
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[Presentation] Identification of mRNAs which move long-distance2014
Author(s)
Michitaka Notaguchi, Takamasa Suzuki, Tetsuya Higashiyama
Organizer
International ERATO Higashiyama Live-Holonics Symposium 2014 "Plant Live-Cell Imaging and Microdevices"
Place of Presentation
愛知県名古屋市, 名古屋大学
Year and Date
2014-09-09 – 2014-09-10
Related Report
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[Presentation] Identification of mRNAs which move long-distance2014
Author(s)
Michitaka Notaguchi, Takamasa Suzuki, Tetsuya Higashiyama
Organizer
Intercellular communication in plant development and disease
Place of Presentation
France, Bischoffsheim, Le Bischenberg Centre de Congres et Seminaires
Year and Date
2014-08-24 – 2014-08-29
Related Report
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