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Study on plant systemic signaling via phloem

Research Project

Project/Area Number 25650095
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNagoya University

Principal Investigator

NOTAGUCHI Michitaka  名古屋大学, 理学(系)研究科(研究院), 研究員 (00647927)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords移動性mRNA / 篩管 / 長距離移行 / 長距離シグナル伝達 / 接木 / 高速シークエンサー
Outline of Final Research Achievements

Phloem is a conductive tissue that allocates nutrients from mature source leaves to sinks such as young developing tissues. Phloem also delivers proteins and RNA species, such as small RNAs and mRNAs. Intensive studies on plant systemic signaling revealed the essential roles for proteins and RNA species. However, many of their functions are still largely unknown. A major difficulty is the absence of an accurate and comprehensive list of mobile transcripts. In this study, we used a hetero-graft system with Nicotiana benthamiana as the recipient scion and Arabidopsis as the donor stock, to identify transcripts that moved long distances across the graft union. We identified 138 Arabidopsis transcripts as mobile mRNAs, which we collectively termed the mRNA mobilome. RT-PCR, qRT-PCR and droplet digital PCR analyses confirmed the mobility. The transcripts included potential signaling factors and, unexpectedly, more general factors.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (12 results) (of which Invited: 6 results)

  • [Journal Article] Identification of mRNAs that Move Over Long Distances Using an RNA-Seq Analysis of Arabidopsis/Nicotiana benthamiana Heterografts2015

    • Author(s)
      Michitaka Notaguchi, Tetsuya Higashiyama, Takamasa Suzuki
    • Journal Title

      Plant Cell Physiology

      Volume: 56 Issue: 2 Pages: 311-321

    • DOI

      10.1093/pcp/pcu210

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of phloem-mobile mRNA2015

    • Author(s)
      Michitaka Notaguchi
    • Journal Title

      Journal of Plant Research

      Volume: 128 Issue: 1 Pages: 27-35

    • DOI

      10.1007/s10265-014-0675-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dynamics of long-distance signaling via plant vascular tissues2015

    • Author(s)
      Michitaka Notaguchi, Satoru Okamoto
    • Journal Title

      Frontiers in Plant Science

      Volume: 18 Pages: 161-161

    • DOI

      10.3389/fpls.2015.00161

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] タバコ属を用いた異科接木の挑戦2015

    • Author(s)
      野田口理孝, 佐藤良勝, 東山哲也
    • Organizer
      園芸学会 平成27年度春季大会
    • Place of Presentation
      千葉県千葉市, 千葉大学
    • Year and Date
      2015-03-28 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ属を用いた異科接木の挑戦2015

    • Author(s)
      野田口理孝, 佐藤良勝, 東山哲也
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      東京都町田市, 玉川大学
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ属を用いた異科接木への挑戦2015

    • Author(s)
      野田口理孝, 佐藤良勝, 東山哲也
    • Organizer
      日本植物生理学会 第56回年会
    • Place of Presentation
      東京都世田谷区, 東京農業大学
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] A challenge to overcome graft-incompatibility using Nicotiana plants2015

    • Author(s)
      Michitaka Notaguchi, Yoshikatsu Sato, Tetsuya Higashiyama
    • Organizer
      第8回国際環境生物資源学会シンポジウム Exploring the global sustainability -Advances in Plant Biotechnology for Agriculture in Semi-arid land-
    • Place of Presentation
      大阪府吹田市, 大阪大学
    • Year and Date
      2015-03-03 – 2015-03-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] A challenge to overcome graft-incompatibility using Nicotiana plants2014

    • Author(s)
      Michitaka Notaguchi
    • Organizer
      Plant reprogramming workshop
    • Place of Presentation
      神奈川県横浜市, 理化学研究所
    • Year and Date
      2014-11-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 篩管を長距離移行する高分子は何をする?2014

    • Author(s)
      野田口理孝
    • Organizer
      平成26年度シンポジウム「植物の中をめぐる多様なシグナル分子」
    • Place of Presentation
      奈良県生駒市, 奈良先端科学技術大学院大学
    • Year and Date
      2014-11-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タバコ属を用いた異科接木への挑戦2014

    • Author(s)
      野田口理孝
    • Organizer
      11th JSOL International Symposium on Solanaceae Genomics
    • Place of Presentation
      愛知県名古屋市, 名古屋大学
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 篩管中を長距離輸送されるmRNAに関する研究2014

    • Author(s)
      野田口理孝, 鈴木孝征, 東山哲也
    • Organizer
      第78回日本植物学会大会
    • Place of Presentation
      神奈川県川崎市, 明治大学
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of mRNAs which move long-distance2014

    • Author(s)
      Michitaka Notaguchi, Takamasa Suzuki, Tetsuya Higashiyama
    • Organizer
      International ERATO Higashiyama Live-Holonics Symposium 2014 "Plant Live-Cell Imaging and Microdevices"
    • Place of Presentation
      愛知県名古屋市, 名古屋大学
    • Year and Date
      2014-09-09 – 2014-09-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of mRNAs which move long-distance2014

    • Author(s)
      Michitaka Notaguchi, Takamasa Suzuki, Tetsuya Higashiyama
    • Organizer
      Intercellular communication in plant development and disease
    • Place of Presentation
      France, Bischoffsheim, Le Bischenberg Centre de Congres et Seminaires
    • Year and Date
      2014-08-24 – 2014-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of phloem-mobile mRNAs through the analysis of hetero-graft samples2014

    • Author(s)
      Michitaka Notaguchi, Takamasa Suzuki, Tetsuya Higashiyama
    • Organizer
      第55回日本植物生理学会大会
    • Place of Presentation
      富山県富山市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 篩管中を移動するRNA2013

    • Author(s)
      野田口理孝
    • Organizer
      第5回植物科学若手研究会
    • Place of Presentation
      愛知県犬山市
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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