Interactome analysis with biotin ligase in Arabidopsis

• Project/Area Number: 25650096
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: TAMURA Kentaro 京都大学, 理学(系)研究科(研究院), 助教 (40378609)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: インタラクトーム / ビオチン / ビオチンライゲース / シロイヌナズナ / BirA / 相互作用

Outline of Final Research Achievements

Interactomics is a powerful resource to reveal the whole set of molecular protein network in the cells. In this study, we aimed to develop a novel tool for interactomics in plant nuclear envelope by using modified biotin ligase (BirA). We generated transgenic Arabidopsis plants expressing BirA-fused nuclear envelope protein. Western blot analyses indicated that the biotinylated proteins are specifically accumulated in the transgenic plants. However, the accumulation level of biotinylated proteins was low. To purify and identify more effectively, it is required for the system allowing higher accumulation of biotinylated proteins in the transgenic plants

Research Products

• [Journal Article] Recent advances in understanding plant nuclear envelope proteins involved in nuclear morphology (2015)
  • Author(s): Kentaro Tamura, Chieko Goto and Ikuko Hara-Nishimura
  • Journal Title: Journal of Experimental Botany Volume: 66 Issue: 6 Pages: 1641-1647
  • DOI: 10.1093/jxb/erv036
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] BEACH-Domain Proteins Act Together in a Cascade to Mediate Vacuolar Protein Trafficking and Disease Resistance in Arabidopsis. (2015)
  • Author(s): Teh OK, Hatsugai N, Tamura K, Fuji K, Tabata R, Yamaguchi K, Shingenobu S, Yamada M, Hasebe M, Sawa S, Shimada T, Hara-Nishimura I.
  • Journal Title: Mol Plant Volume: 8 Issue: 3 Pages: 389-398
  • DOI: 10.1016/j.molp.2014.11.015
  • NAID: 120005646465
  • Peer Reviewed / Open Access
• [Journal Article] Functional insights of nucleocytoplasmic transport in plants (2014)
  • Author(s): Tamura K, Hara-Nishimura I
  • Journal Title: Front Plant Sci Volume: 5 Pages: 1-10
  • DOI: 10.3389/fpls.2014.00118
  • NAID: 120005439543
  • Peer Reviewed / Open Access
• [Journal Article] The Novel Nuclear Envelope Protein KAKU4 Modulates Nuclear Morphology in Arabidopsis (2014)
  • Author(s): Goto C, Tamura K, Fukao Y, Shimada T, Hara-Nishimura I.
  • Journal Title: Plant Cell Volume: 26 Issue: 5 Pages: 2143-2155
  • DOI: 10.1105/tpc.113.122168
  • Peer Reviewed / Open Access
• [Journal Article] GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid accumulation in Arabidopsis thaliana (2014)
  • Author(s): Ichino T, Fuji K, Ueda H, Takahashi H, Koumoto Y, Takagi J, Tamura K, Sasaki R, Aoki K, Shimada T, Hara-Nishimura I.
  • Journal Title: Plant J Volume: 80 Issue: 3 Pages: 410-423
  • DOI: 10.1111/tpj.12637
  • Peer Reviewed / Open Access
• [Journal Article] Nucleoporin 75, a component of nuclear pore complex, is involved in the MAPK-mediated production of ethylene leading to the accumulation of phytoalexin for the resistance of Nicotiana benthamiana to Phytophthora infestans (2014)
  • Author(s): Ohtsu et al.
  • Journal Title: Mol. Plant Microbe Interact. Volume: 27 Issue: 12 Pages: 1318-1330
  • DOI: 10.1094/mpmi-06-14-0181-r
  • Peer Reviewed / Open Access
• [Journal Article] MAIGO5 Functions in Protein Export from Golgi-Associated Endoplasmic Reticulum Exit sites in Arabidopsis. (2013)
  • Author(s): Junpei Takagi, Luciana Renna, Hideyuki Takahashi, Yasuko Koumoto, Kentaro Tamura, Giovanni Stefano, Yoichiro Fukao, Maki Kondo, Mikio Nishimura, Tomoo Shimada, Federica Brandizzi, Ikuko Hara-Nishimura
  • Journal Title: The Plant Cell Volume: 25(11) Issue: 11 Pages: 4658-75
  • DOI: 10.1105/tpc.113.118158
  • Peer Reviewed / Open Access
• [Journal Article] A myosin XI-I Links the Nuclear Membrane with Cytoskeleton for Nuclear Movement and Shape in Arabidopsis thaliana (2013)
  • Author(s): Tamura K, Iwabuchi K, Fukao Y, Kondo M, Okamoto K, Ueda H, Nishimura M, Hara-Nishimura I
  • Journal Title: Current Biology Volume: 23 Issue: 18 Pages: 1776-1781
  • DOI: 10.1016/j.cub.2013.07.035
  • Peer Reviewed
• [Journal Article] The molecular architecture of the plant nuclear pore complex. (2013)
  • Author(s): Tamura K et al.
  • Journal Title: J Exp Bot. Volume: 64(4) Issue: 4 Pages: 823-832
  • DOI: 10.1093/jxb/ers258
  • NAID: 120005324912
  • Peer Reviewed
• [Presentation] Interactive proteomics dissects structural dynamics of plant nuclear envelope. (2014)
  • Author(s): Tamura et al.
  • Organizer: 1st International Plant Proteomics Organisation World Congress
  • Place of Presentation: Hamburg, Germany
  • Year and Date: 2014-08-31 – 2014-09-04
  • Invited
• [Presentation] Key regulators of nuclear structure and dynamics in Arabidopsis. (2014)
  • Author(s): Kentaro Tamura
  • Organizer: Society for Experimental Biology Annual Main Meeting
  • Place of Presentation: Manchester, United Kingdom
  • Year and Date: 2014-07-01 – 2014-07-04
  • Invited
• [Presentation] 植物の核膜がもつ新しい役割 (2014)
  • Author(s): 田村ら
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学
• [Presentation] Characterisation of a novel nucleoporin and plant specific nuclear envelope proteins (2013)
  • Author(s): Tamura et al.
  • Organizer: 1st IPNC
  • Place of Presentation: Oxford Brookes University, UK.
• [Remarks] 植物学教室年報ー植物分子細胞生物学分科
  • URL: http://www.bot.kyoto-u.ac.jp/annual/4_saibou.html
• [Remarks] 細胞核の運動のしくみの解明 －植物固有のナノマシンの発見－
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130823_1.htm