Parental effects on embryogenesis of Arabidopsis thaliana

• Project/Area Number: 25650100
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: FURUTANI MASAHIKO 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10432593)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 植物胚発生 / 配偶子効果 / 卵細胞 / 受精卵 / RNAの可視化 / 細胞特異的プロモーター / 初期胚 / RNA結合タンパク質

Outline of Final Research Achievements

I analyzed the function of the paternal-effect gene SSP1 and maternal-effect gene AtMAGO in apical-basal axis formation of Arabidopsis embryo to uncover the molecular mechanisms of parental effects on plant embryogenesis. I found that AtMAGO is required for the proper polarized-localization of SSP1 in the zygote and that AtMAGO is also involved in SSP1-driven MAPKKK signaling cascade. Besides, RNA-binding protein AtMAGO was localized in the periphery of egg cell with polarity, suggesting apical-basal axis formation in early embryogenesis via polar-localized SSP1 mRNA.

Research Products

• [Journal Article] Hayashi K, Nakamura S, Fukunaga S, Nishimura T, Jenness MK, Murphy AS, Motose H, Nozaki H, Furutani M, Aoyama T. (2014)
  • Author(s): Auxin transport sites are visualized in planta using fluorescent auxin analogs.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 111 Issue: 31 Pages: 111557-111562
  • DOI: 10.1073/pnas.1408960111
  • Peer Reviewed / Open Access
• [Journal Article] The CUC1 and CUC2 genes promote carpel margin meristem formation during Arabidopsis gynoecium development (2014)
  • Author(s): Kamiuchi Y, Yamamoto K, Furutani M,Tasaka M, Aida M
  • Journal Title: Frontiers in Plant Science Volume: 5 Pages: 165-165
  • DOI: 10.3389/fpls.2014.00165
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] MAB4-induced auxin sink generates local auxin gradients in Arabidopsis organ formation (2014)
  • Author(s): Masahiko Furutani, Yasukazu Nakano, Masao Tasaka
  • Journal Title: Proc Natl Acad Sci U S A Volume: 113 Issue: 3 Pages: 1198-1203
  • DOI: 10.1073/pnas.1316109111
  • NAID: 120005716843
  • Peer Reviewed
• [Presentation] Mitochondria-derived reactive oxygen species are required for polar auxin transport in Arabidopsis organ formation (2015)
  • Author(s): Furutani M, Tasaka M
  • Organizer: International Conference for Plant Mitochondrial Biology
  • Place of Presentation: マーキュリーホテル ヴロツワフ（ポーランド・ヴロツワフ）
  • Year and Date: 2015-05-18 – 2015-05-19
• [Presentation] Mitochondria-derived reactive oxygen species are required for polar auxin transport in Arabidopsis organ formation. (2015)
  • Author(s): Furutani M., Tasaka M.
  • Organizer: 9th International Conference for Plant Mitochondrial Biology
  • Place of Presentation: Wroclaw, Poland
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] The MAB4-PID signaling complex controls PIN dynamics on the plasma membrane (2015)
  • Author(s): Furutani M, Tasaka M
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都世田谷区）
  • Year and Date: 2015-03-18
• [Presentation] The MAB4-PID signaling complex controls PIN dynamics on the plasma membrane (2015)
  • Author(s): Furutani M, Tasaka M
  • Organizer: Plant Organ Growth Symposium
  • Place of Presentation: ゲント大（ベルギー・ゲント）
  • Year and Date: 2015-03-11 – 2015-03-12
• [Presentation] The MAB4-PID signaling complex controls PIN dynamics on the plasma membrane (2014)
  • Author(s): Furutani M, Tasaka M
  • Organizer: The 5th NIBB-MPIPZ-TLL合同シンポジウム
  • Place of Presentation: マックスプランク研究所（ドイツ・ケルン）
  • Year and Date: 2014-11-25 – 2014-11-26
• [Presentation] The MAB4-PID signaling complex controls PIN dynamics on the plasma membrane (2014)
  • Author(s): Furutani M, Tasaka M
  • Organizer: 内藤コンファレンス「Molecule-based biological systems」
  • Place of Presentation: シャトレーゼ ガトーキングダム サッポロ（北海道札幌市）
  • Year and Date: 2014-10-08 – 2014-10-09
• [Presentation] Auxin response links two types of PIN1 polarization in phyllotactic organogenesis (2014)
  • Author(s): Masahiko Furutani, Yasukazu Nakano, Masao Tasaka
  • Organizer: 第５５回日本植物生理学会年会
  • Place of Presentation: 富山大学 五福キャンパス、富山県富山市
  • Invited
• [Presentation] 器官発生過程におけるオーキシン極性輸送制御機構 (2013)
  • Author(s): 古谷 将彦、中野 泰一、田坂 昌生
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 北海道大学 札幌キャンパス、北海道札幌市
  • Invited
• [Presentation] PINタンパク質の細胞膜近傍の動態制御 (2013)
  • Author(s): 古谷 将彦、阪本 展仁、田坂 昌生
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 北海道大学 札幌キャンパス、北海道札幌市