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Comprehensive understanding of organelle division and multiplication on bases of elucidation of dividing machinery of microbody

Research Project

Project/Area Number 25650101
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionRikkyo University

Principal Investigator

KUROIWA Tsuneyoshi  立教大学, 理学部, 特定課題研究員 (50033353)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsマイクロボディ / ペルオキシソーム / 膜系オルガネラ / 単膜系オルガネラ / 複膜系オルガネラ / 分裂マシン / ダイナミン / オルガネラ分裂 / シゾン / プロテオーム / ミトコンドリア分裂 / 葉緑体分裂
Outline of Final Research Achievements

The double-membrane-bounded and the single-membrane-bounded organelles are required to fulfill the functions of eukaryotic cells. Cyanidioschyzon merolae cells contain a minimum set of essential organelles and their cell cycles are synchronized by light and dark cycles. We found the dividing machineries (MD and PD) in double-membrane-bounded mitochondria and plastids.
In this study, we have found using omics research that a single-membrane-bounded organelle, microbody (peroxisome), divides using a complex of outer ring (POD) containing dynamin (similar to mitochondrial dynamin) and inner ring composed of fine filaments, and proposed a molecular model of microbody machinery. Finally we compared POD, MD and PD.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (39 results)

All 2015 2014 2013 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (17 results) (of which Invited: 5 results) Book (7 results) Remarks (3 results)

  • [Journal Article] Real Time Imaging of Biological Phenomena with Super-duper Luminescent Proteins2015

    • Author(s)
      Sakai A, Takusagawa M, Nio A, Sawai Y
    • Journal Title

      CYTOLOGIA

      Volume: 80 Issue: 1 Pages: 1-2

    • DOI

      10.1508/cytologia.80.1

    • NAID

      130004801668

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimization of polyethylene glycol (PEG)-mediated DNA introduction conditions for transient gene expression in the unicellular red alga <i>Cyanidioschyzon merolae</i>2014

    • Author(s)
      Mio Ohnuma, Takashi Yokoyama, Takayuki Inouye, Yasuhiko Sekine, Tsuneyoshi Kuroiwa and Kan Tanaka
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 60 Issue: 4 Pages: 156-159

    • DOI

      10.2323/jgam.60.156

    • NAID

      130004694351

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparative analysis of zygospore transcripts during early germination in Chlamydomonas reinhardtii2014

    • Author(s)
      Aoyama, H., Saitoh, S., Kuroiwa, T. and Nakamura, S.
    • Journal Title

      J Plant Physiol.

      Volume: 171 Issue: 18 Pages: 1685-1692

    • DOI

      10.1016/j.jplph.2014.07.016

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Algae sense exact temperatures: small heat shock proteins are expressed at the survival threshold temperature in Cyanidioschyzon merolae and Chlamydomonas reinhardtii.2014

    • Author(s)
      Kobayashi Y, Harada N, Nishimura Y, Saito T, Nakamura M, Fujiwara T, Kuroiwa T, Misumi O.
    • Journal Title

      Genome Biol Evol.

      Volume: 6 Issue: 10 Pages: 2731-2740

    • DOI

      10.1093/gbe/evu216

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lipid Droplet Formation in Cells of the Filamentous Green Alga <i>Klebsormidium nitens</i> as Revealed by BODIOY-DiOC<sub>6</sub> and BODIPY-Nile Red Double-Staining Microscopy2014

    • Author(s)
      Kuroiwa T, Ohnuma M, Imoto Y, and Kuroiwa H
    • Journal Title

      CYTOLOGIA

      Volume: 79 Issue: 4 Pages: 501-507

    • DOI

      10.1508/cytologia.79.501

    • NAID

      130004801653

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chronological transition of mitochondrial morphology in long-term culture of Chlamydomonas einhardtii.2013

    • Author(s)
      Aoyama H., Kuroiwa T, Nakamura S
    • Journal Title

      J Phycol

      Volume: 49 Issue: 2 Pages: 340-348

    • DOI

      10.1111/jpy.12040

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Golgi inheritance in the primitive red alga, Cyanidioschyzon merolae2013

    • Author(s)
      Fumi Yagisawa, Takayuki Fujiwara, Mio Ohnuma, Keiji Nishida, Yuuta Imoto, Yamato Yoshida, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
    • Journal Title

      Protoplasma

      Volume: 250 Issue: 4 Pages: 943-948

    • DOI

      10.1007/s00709-012-0467-6

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of highly-disrupted tRNAs in nuclear genome of the red alga, Cyanidioschyzon merolae 10D.2013

    • Author(s)
      Akiko Soma, Junichi Sugahara, Akinori Onodera, Nozomu Yachie, Akio Kanai, Satoru Watanabe, Hirofumi Yoshikawa, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, and Yasuhiko Sekine
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 2321-2329

    • DOI

      10.1038/srep02321

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The kineain-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division2013

    • Author(s)
      Yamato Yoshida, Takayuki Fujiwara, Yuuta Imoto, Masaki Yoshida, Mio Ohnuma, Shunsuke Hirooka, Osami Misumi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
    • Journal Title

      Journal of Cell Science

      Volume: 126 Pages: 2392-2400

    • DOI

      10.1242/jcs.116798

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery2013

    • Author(s)
      Yuuta Imoto, Haruko Kuroiwa, Yamato Yoshida, Mio Ohnuma, Takayuki Fujiwara, Masaki Yoshida, Keiji Nishida, Fumi Yagisawa, Shunsuke Hirooka, Shinya Miyagishima, Osami Misumi, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 110 Issue: 23 Pages: 9583-9588

    • DOI

      10.1073/pnas.1303483110

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal dynamics of condensins I and II : evolutionary insights from the primitive red alga Cyanidioschyzon merolae.2013

    • Author(s)
      Fujiwara T, Tanaka K, Kuroiwa T, Hirano T.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 24 Issue: 16 Pages: 2515-2527

    • DOI

      10.1091/mbc.e13-04-0208

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gene targeting in the red alga Cyanidioschyzon merolae : single- and multi-copy insertion using authentic and chimeric selection markers.2013

    • Author(s)
      Fujiwara T, Ohnuma M, Yoshida M, Kuroiwa T, Hirano T.
    • Journal Title

      PLoS One

      Volume: 8 Issue: 9 Pages: e73608-e73608

    • DOI

      10.1371/journal.pone.0073608

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 真核生物の普遍原理の探求―原核生物より小さな真核生物を基盤に2014

    • Author(s)
      黒岩常祥
    • Organizer
      東京大学生物科学専攻統合記念シンポジウム
    • Place of Presentation
      東京都 文京区
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] シゾンの形質転換系を用いた高温耐性遺伝子過剰発現の効果2014

    • Author(s)
      大沼みお、藤原崇之、井元祐太、廣岡俊亮、黒岩晴子、三角修己、黒岩常祥
    • Organizer
      日本植物学会 第78回大会
    • Place of Presentation
      神奈川 川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 藻類におけるバイオエネルギー生産に関わる機能を向上させる培地の探索2014

    • Author(s)
      黒岩晴子、大沼みお、三角修己、井元祐太、黒岩常祥
    • Organizer
      日本植物学会 第78回大会
    • Place of Presentation
      神奈川 川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] バイオ燃料産生におけるオルガネラの役割2014

    • Author(s)
      黒岩常祥、大沼みお、三角修己、井元祐太、黒岩晴子
    • Organizer
      日本植物学会 第78回大会
    • Place of Presentation
      神奈川 川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ポストゲノミクスを基盤としたペルオキシソーム分裂装置の構造と機能解析2014

    • Author(s)
      井元祐太、黒岩晴子、大沼みお、藤木幸夫、黒岩常祥
    • Organizer
      日本植物学会 第78回大会
    • Place of Presentation
      神奈川 川崎市
    • Year and Date
      2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 単膜系オルガネラ分裂リングの同定 - ゲノム科学を基盤としたペルオキシソーム分裂装置(POD machinery)の微細構造と分子機構の解析2014

    • Author(s)
      井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、河野重行、黒岩常祥
    • Organizer
      日本細胞生物学会大会
    • Place of Presentation
      奈良県 奈良市
    • Year and Date
      2014-06-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 20億年前に誕生した細胞からあなたまで、そして未来へ2014

    • Author(s)
      黒岩常祥
    • Organizer
      日本学士院第六十回公開講演会
    • Place of Presentation
      山梨県 甲府市
    • Year and Date
      2014-05-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞分裂に隠された細胞誕生のしくみー三つの細胞小器官の分裂装置の発見から

    • Author(s)
      黒岩常祥
    • Organizer
      第14回IGERグリーン自然科学レクチャー
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] オルガネラの分裂・増殖の仕組みから観た生物の誕生・現在・未来

    • Author(s)
      黒岩常祥
    • Organizer
      神谷宣郎生誕100周年記念シンポジウム
    • Place of Presentation
      大阪大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける遺伝子改変技術の改良とシアニジウム類における高温耐性株選択法

    • Author(s)
      大沼みお、井元祐太、黒岩晴子、黒岩常祥
    • Organizer
      日本植物形態学会第25回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 原始紅藻シゾンにおけるペルオキシソーム分裂装置 (Pod-machinery) の構造と分子機構の解明

    • Author(s)
      井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、黒岩常祥、河野重行
    • Organizer
      日本植物形態学会第25回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] シゾンにおける遺伝子改変技術の改良とシアニジウム類における高温耐性株選択法

    • Author(s)
      大沼みお、井元祐太、黒岩晴子、三角修己、兼崎 友、黒岩常祥
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 藻類における細胞小器官の脂質合成に関わる機能の細胞学的研究

    • Author(s)
      黒岩晴子、三角修己、大沼みお、井元祐太、黒岩常祥
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 単膜系細胞小器官の分裂装置から見た真核細胞の起源~ミトコンドリアとペルオキシソームは兄弟~

    • Author(s)
      黒岩常祥、井元祐太、黒岩晴子、大沼みお
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] ポストゲノミクスを基盤としたペルオキシソーム分裂装置(Pod-machinery)の構造同定と分子機構の解明

    • Author(s)
      井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、黒岩常祥、河野重行
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      札幌 北海道大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeの分子遺伝学的手法の開発とその応用

    • Author(s)
      大沼みお、吉田大和、藤原崇之、井元祐太、八木沢芙美、今村壮輔、三角修己、吉田昌樹、黒岩晴子、田中寛、黒岩常祥
    • Organizer
      第7回 日本ゲノム微生物学会若手の会
    • Place of Presentation
      静岡県駿東郡ろうきん研修所富士センター
    • Related Report
      2013 Research-status Report
  • [Presentation] On the origin of organelles: Why is mitochondrial division accompanied by inheritance of peroxisome and lysosome? The 4th International symposium on dynamics of mitochondria from molecular mechanisms to physiological function and diseases

    • Author(s)
      Tsuneyoshi Kuroiwa
    • Organizer
      The 4th International symposium on dynamics of mitochondria
    • Place of Presentation
      Okinawa,残波岬ロイヤルホテル
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] Mechanisms of division and inheritance of mitochondria and chroroplastsin Atlas in Plant Cell Structure2014

    • Author(s)
      Kuroiwa, T. and Miyakawa, I. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Chloroplast divide by contraction of a bundle of polyglucan nanofilaments in Atlas in Plant Cell Structure2014

    • Author(s)
      Yoshida, Y.and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Chloroplast division machinery in Pelargonium zonale Ait in Atlas in Plant Cell Structure2014

    • Author(s)
      Kuroiwa, H. and Kuroiwa, T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Production of oil bodies in response to nitogen starvation in Chlamudomonas reinhardtii in IAtlas in Plant Cell Structure2014

    • Author(s)
      Kuroiwa, H. and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Egg cells with giant mitochondria in a higher plant, Pelargonium zonale Ait in Atlas in Plant Cell Structure2014

    • Author(s)
      Kuroiwa, H. and Kuroiwa,T(eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] AZygote and sperm cells during early embryogenesis in higher plant, Pelargonium zonale Ait iin Atlas in Plant Cell Structure2014

    • Author(s)
      Kuroiwa, H. and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
    • Total Pages
      202
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Atlas of Plan Cell Structure2014

    • Author(s)
      Kuroiwa H. and Kuroiwa, T (ed. Noguchi T.,Kawano,S. et al.)
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report
  • [Remarks] 立教大学HPニュース: 真核細胞誕生の謎を解く ~ペルオキシソームに、第三の分裂装置~

    • URL

      http://www.rikkyo.ac.jp/news/

    • Related Report
      2013 Research-status Report
  • [Remarks] 立教大学HPニュース: 細胞分裂の基本的しくみ解明 、全細胞小器官の分裂を統御する遺伝子発見

    • URL

      http://www.rikkyo.ac.jp/news/

    • Related Report
      2013 Research-status Report
  • [Remarks] 立教大学HPニュース: 真核生物のゲノム複製の基本となる染色体の分配のしくみを解明

    • URL

      http://www.rikkyo.ac.jp/news/

    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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