Comprehensive understanding of organelle division and multiplication on bases of elucidation of dividing machinery of microbody

• Project/Area Number: 25650101
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Rikkyo University
• Principal Investigator: KUROIWA Tsuneyoshi 立教大学, 理学部, 特定課題研究員 (50033353)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マイクロボディ / ペルオキシソーム / 膜系オルガネラ / 単膜系オルガネラ / 複膜系オルガネラ / 分裂マシン / ダイナミン / オルガネラ分裂 / シゾン / プロテオーム / ミトコンドリア分裂 / 葉緑体分裂

Outline of Final Research Achievements

The double-membrane-bounded and the single-membrane-bounded organelles are required to fulfill the functions of eukaryotic cells. Cyanidioschyzon merolae cells contain a minimum set of essential organelles and their cell cycles are synchronized by light and dark cycles. We found the dividing machineries (MD and PD) in double-membrane-bounded mitochondria and plastids.
In this study, we have found using omics research that a single-membrane-bounded organelle, microbody (peroxisome), divides using a complex of outer ring　(POD) containing dynamin (similar to mitochondrial dynamin) and inner ring composed of fine filaments, and proposed a molecular model of microbody machinery. Finally we compared POD, MD and PD.

Research Products

• [Journal Article] Real Time Imaging of Biological Phenomena with Super-duper Luminescent Proteins (2015)
  • Author(s): Sakai A, Takusagawa M, Nio A, Sawai Y
  • Journal Title: CYTOLOGIA Volume: 80 Issue: 1 Pages: 1-2
  • DOI: 10.1508/cytologia.80.1
  • NAID: 130004801668
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access
• [Journal Article] Optimization of polyethylene glycol (PEG)-mediated DNA introduction conditions for transient gene expression in the unicellular red alga <i>Cyanidioschyzon merolae</i> (2014)
  • Author(s): Mio Ohnuma, Takashi Yokoyama, Takayuki Inouye, Yasuhiko Sekine, Tsuneyoshi Kuroiwa and Kan Tanaka
  • Journal Title: The Journal of General and Applied Microbiology Volume: 60 Issue: 4 Pages: 156-159
  • DOI: 10.2323/jgam.60.156
  • NAID: 130004694351
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparative analysis of zygospore transcripts during early germination in Chlamydomonas reinhardtii (2014)
  • Author(s): Aoyama, H., Saitoh, S., Kuroiwa, T. and Nakamura, S.
  • Journal Title: J Plant Physiol. Volume: 171 Issue: 18 Pages: 1685-1692
  • DOI: 10.1016/j.jplph.2014.07.016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Algae sense exact temperatures: small heat shock proteins are expressed at the survival threshold temperature in Cyanidioschyzon merolae and Chlamydomonas reinhardtii. (2014)
  • Author(s): Kobayashi Y, Harada N, Nishimura Y, Saito T, Nakamura M, Fujiwara T, Kuroiwa T, Misumi O.
  • Journal Title: Genome Biol Evol. Volume: 6 Issue: 10 Pages: 2731-2740
  • DOI: 10.1093/gbe/evu216
  • Peer Reviewed / Open Access
• [Journal Article] Lipid Droplet Formation in Cells of the Filamentous Green Alga <i>Klebsormidium nitens</i> as Revealed by BODIOY-DiOC₆ and BODIPY-Nile Red Double-Staining Microscopy (2014)
  • Author(s): Kuroiwa T, Ohnuma M, Imoto Y, and Kuroiwa H
  • Journal Title: CYTOLOGIA Volume: 79 Issue: 4 Pages: 501-507
  • DOI: 10.1508/cytologia.79.501
  • NAID: 130004801653
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access
• [Journal Article] Chronological transition of mitochondrial morphology in long-term culture of Chlamydomonas einhardtii. (2013)
  • Author(s): Aoyama H., Kuroiwa T, Nakamura S
  • Journal Title: J Phycol Volume: 49 Issue: 2 Pages: 340-348
  • DOI: 10.1111/jpy.12040
  • Peer Reviewed
• [Journal Article] Golgi inheritance in the primitive red alga, Cyanidioschyzon merolae (2013)
  • Author(s): Fumi Yagisawa, Takayuki Fujiwara, Mio Ohnuma, Keiji Nishida, Yuuta Imoto, Yamato Yoshida, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
  • Journal Title: Protoplasma Volume: 250 Issue: 4 Pages: 943-948
  • DOI: 10.1007/s00709-012-0467-6
  • Peer Reviewed
• [Journal Article] Identification of highly-disrupted tRNAs in nuclear genome of the red alga, Cyanidioschyzon merolae 10D. (2013)
  • Author(s): Akiko Soma, Junichi Sugahara, Akinori Onodera, Nozomu Yachie, Akio Kanai, Satoru Watanabe, Hirofumi Yoshikawa, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, and Yasuhiko Sekine
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 2321-2329
  • DOI: 10.1038/srep02321
  • Peer Reviewed
• [Journal Article] The kineain-like protein TOP promotes Aurora localisation and induces mitochondrial, chloroplast and nuclear division (2013)
  • Author(s): Yamato Yoshida, Takayuki Fujiwara, Yuuta Imoto, Masaki Yoshida, Mio Ohnuma, Shunsuke Hirooka, Osami Misumi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa
  • Journal Title: Journal of Cell Science Volume: 126 Pages: 2392-2400
  • DOI: 10.1242/jcs.116798
  • Peer Reviewed
• [Journal Article] Single-membrane-bounded peroxisome division revealed by isolation of dynamin-based machinery (2013)
  • Author(s): Yuuta Imoto, Haruko Kuroiwa, Yamato Yoshida, Mio Ohnuma, Takayuki Fujiwara, Masaki Yoshida, Keiji Nishida, Fumi Yagisawa, Shunsuke Hirooka, Shinya Miyagishima, Osami Misumi, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110 Issue: 23 Pages: 9583-9588
  • DOI: 10.1073/pnas.1303483110
  • Peer Reviewed
• [Journal Article] Spatiotemporal dynamics of condensins I and II : evolutionary insights from the primitive red alga Cyanidioschyzon merolae. (2013)
  • Author(s): Fujiwara T, Tanaka K, Kuroiwa T, Hirano T.
  • Journal Title: Molecular Biology of the Cell Volume: 24 Issue: 16 Pages: 2515-2527
  • DOI: 10.1091/mbc.e13-04-0208
  • Peer Reviewed
• [Journal Article] Gene targeting in the red alga Cyanidioschyzon merolae : single- and multi-copy insertion using authentic and chimeric selection markers. (2013)
  • Author(s): Fujiwara T, Ohnuma M, Yoshida M, Kuroiwa T, Hirano T.
  • Journal Title: PLoS One Volume: 8 Issue: 9 Pages: e73608-e73608
  • DOI: 10.1371/journal.pone.0073608
  • Peer Reviewed
• [Presentation] 真核生物の普遍原理の探求―原核生物より小さな真核生物を基盤に (2014)
  • Author(s): 黒岩常祥
  • Organizer: 東京大学生物科学専攻統合記念シンポジウム
  • Place of Presentation: 東京都 文京区
  • Year and Date: 2014-09-27
  • Invited
• [Presentation] シゾンの形質転換系を用いた高温耐性遺伝子過剰発現の効果 (2014)
  • Author(s): 大沼みお、藤原崇之、井元祐太、廣岡俊亮、黒岩晴子、三角修己、黒岩常祥
  • Organizer: 日本植物学会 第７８回大会
  • Place of Presentation: 神奈川 川崎市
  • Year and Date: 2014-09-12
• [Presentation] 藻類におけるバイオエネルギー生産に関わる機能を向上させる培地の探索 (2014)
  • Author(s): 黒岩晴子、大沼みお、三角修己、井元祐太、黒岩常祥
  • Organizer: 日本植物学会 第７８回大会
  • Place of Presentation: 神奈川 川崎市
  • Year and Date: 2014-09-12
• [Presentation] バイオ燃料産生におけるオルガネラの役割 (2014)
  • Author(s): 黒岩常祥、大沼みお、三角修己、井元祐太、黒岩晴子
  • Organizer: 日本植物学会 第７８回大会
  • Place of Presentation: 神奈川 川崎市
  • Year and Date: 2014-09-12
• [Presentation] ポストゲノミクスを基盤としたペルオキシソーム分裂装置の構造と機能解析 (2014)
  • Author(s): 井元祐太、黒岩晴子、大沼みお、藤木幸夫、黒岩常祥
  • Organizer: 日本植物学会 第７８回大会
  • Place of Presentation: 神奈川 川崎市
  • Year and Date: 2014-09-12
• [Presentation] 単膜系オルガネラ分裂リングの同定 - ゲノム科学を基盤としたペルオキシソーム分裂装置(POD machinery)の微細構造と分子機構の解析 (2014)
  • Author(s): 井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、河野重行、黒岩常祥
  • Organizer: 日本細胞生物学会大会
  • Place of Presentation: 奈良県 奈良市
  • Year and Date: 2014-06-11
• [Presentation] 20億年前に誕生した細胞からあなたまで、そして未来へ (2014)
  • Author(s): 黒岩常祥
  • Organizer: 日本学士院第六十回公開講演会
  • Place of Presentation: 山梨県 甲府市
  • Year and Date: 2014-05-24
  • Invited
• [Presentation] 細胞分裂に隠された細胞誕生のしくみー三つの細胞小器官の分裂装置の発見から
  • Author(s): 黒岩常祥
  • Organizer: 第14回IGERグリーン自然科学レクチャー
  • Place of Presentation: 名古屋大学
  • Invited
• [Presentation] オルガネラの分裂・増殖の仕組みから観た生物の誕生・現在・未来
  • Author(s): 黒岩常祥
  • Organizer: 神谷宣郎生誕100周年記念シンポジウム
  • Place of Presentation: 大阪大学
  • Invited
• [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおける遺伝子改変技術の改良とシアニジウム類における高温耐性株選択法
  • Author(s): 大沼みお、井元祐太、黒岩晴子、黒岩常祥
  • Organizer: 日本植物形態学会第25回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] 原始紅藻シゾンにおけるペルオキシソーム分裂装置 (Pod-machinery) の構造と分子機構の解明
  • Author(s): 井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、黒岩常祥、河野重行
  • Organizer: 日本植物形態学会第25回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] シゾンにおける遺伝子改変技術の改良とシアニジウム類における高温耐性株選択法
  • Author(s): 大沼みお、井元祐太、黒岩晴子、三角修己、兼崎 友、黒岩常祥
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] 藻類における細胞小器官の脂質合成に関わる機能の細胞学的研究
  • Author(s): 黒岩晴子、三角修己、大沼みお、井元祐太、黒岩常祥
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] 単膜系細胞小器官の分裂装置から見た真核細胞の起源～ミトコンドリアとペルオキシソームは兄弟～
  • Author(s): 黒岩常祥、井元祐太、黒岩晴子、大沼みお
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] ポストゲノミクスを基盤としたペルオキシソーム分裂装置(Pod-machinery)の構造同定と分子機構の解明
  • Author(s): 井元祐太、黒岩晴子、吉田大和、大沼みお、藤原崇之、吉田昌樹、西田敬二、八木沢芙美、廣岡俊亮、宮城島進也、三角修己、黒岩常祥、河野重行
  • Organizer: 日本植物学会第７７回大会
  • Place of Presentation: 札幌 北海道大学
• [Presentation] 単細胞紅藻Cyanidioschyzon merolaeの分子遺伝学的手法の開発とその応用
  • Author(s): 大沼みお、吉田大和、藤原崇之、井元祐太、八木沢芙美、今村壮輔、三角修己、吉田昌樹、黒岩晴子、田中寛、黒岩常祥
  • Organizer: 第7回 日本ゲノム微生物学会若手の会
  • Place of Presentation: 静岡県駿東郡ろうきん研修所富士センター
• [Presentation] On the origin of organelles: Why is mitochondrial division accompanied by inheritance of peroxisome and lysosome? The 4th International symposium on dynamics of mitochondria from molecular mechanisms to physiological function and diseases
  • Author(s): Tsuneyoshi Kuroiwa
  • Organizer: The 4th International symposium on dynamics of mitochondria
  • Place of Presentation: Okinawa,残波岬ロイヤルホテル
  • Invited
• [Book] Mechanisms of division and inheritance of mitochondria and chroroplastsin Atlas in Plant Cell Structure (2014)
  • Author(s): Kuroiwa, T. and Miyakawa, I. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] Chloroplast divide by contraction of a bundle of polyglucan nanofilaments in Atlas in Plant Cell Structure (2014)
  • Author(s): Yoshida, Y.and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] Chloroplast division machinery in Pelargonium zonale Ait in Atlas in Plant Cell Structure (2014)
  • Author(s): Kuroiwa, H. and Kuroiwa, T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] Production of oil bodies in response to nitogen starvation in Chlamudomonas reinhardtii in IAtlas in Plant Cell Structure (2014)
  • Author(s): Kuroiwa, H. and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] Egg cells with giant mitochondria in a higher plant, Pelargonium zonale Ait in Atlas in Plant Cell Structure (2014)
  • Author(s): Kuroiwa, H. and Kuroiwa,T(eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] AZygote and sperm cells during early embryogenesis in higher plant, Pelargonium zonale Ait iin Atlas in Plant Cell Structure (2014)
  • Author(s): Kuroiwa, H. and Kuroiwa,T. (eds: Noguchi, T., Kawano, S., Tsukaya, H., Matsunaga, S., Sakai, A., Karahara, I., Hayashi, Y.)
  • Total Pages: 202
  • Publisher: Springer
• [Book] Atlas of Plan Cell Structure (2014)
  • Author(s): Kuroiwa H. and Kuroiwa, T (ed. Noguchi T.,Kawano,S. et al.）
  • Publisher: Springer
• [Remarks] 立教大学HPニュース: 真核細胞誕生の謎を解く ～ペルオキシソームに、第三の分裂装置～
  • URL: http://www.rikkyo.ac.jp/news/
• [Remarks] 立教大学HPニュース: 細胞分裂の基本的しくみ解明 、全細胞小器官の分裂を統御する遺伝子発見
  • URL: http://www.rikkyo.ac.jp/news/
• [Remarks] 立教大学HPニュース: 真核生物のゲノム複製の基本となる染色体の分配のしくみを解明
  • URL: http://www.rikkyo.ac.jp/news/