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Role of commensal bacteria in the switch of reproductive modes of planarians

Research Project

Project/Area Number 25650103
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphology/Structure
Research InstitutionTsuyama National College of Technology

Principal Investigator

MAEZAWA TAKANOBU  津山工業高等専門学校, その他部局等, 准教授 (90548398)

Co-Investigator(Renkei-kenkyūsha) KAZUYA KOBAYASHI  弘前大学, 農学生命科学部, 准教授 (50360110)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsプラナリア / 生殖様式転換 / 共生細菌 / 有性化 / 細菌
Outline of Final Research Achievements

In the process of conversion from asexual to sexual reproduction (sexual induction), hermaphroditic sexual organs are gradually developed in the planarian Dugesia ryukyuensis. Tryptophan (Trp) has been identified as an inducing factor of ovary, which is firstly developed during the sexual induction. Sexual planarians contain higher concentration of Trp than that of asexual planarians. We expected that symbiotic bacteria are concerned with increasing concentration of Trp, an essential amino acid, during sexual induction. In this study, the experiment of treatment of an antibiotic suggested that the bacteria are required for ovarian development at the initial step of sexual induction. Moreover, by comparing bacteria species between asexual and sexual planarians, we found that the rate of a particular bacterium become increased during sexual induction.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2016 2015 2014 Other

All Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results)

  • [Presentation] トリプトファンはDr-SLC38A9の発現誘導を介してプラナリアの有性化を進行させる2015

    • Author(s)
      前澤孝信, 石川正樹, 小林一也
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Tryptophan enhances the reproductive organs-specific expression level of an amino acid transporter homolog, Dr-SLC38A9 to promote sexual induction of the planarian Dugesia ryukyuensis2015

    • Author(s)
      Maezawa T, Ishikawa M, Nagamatsu G and Kobayashi K
    • Organizer
      International planarian/regeneration meeting
    • Place of Presentation
      Oxford, United Kingdom
    • Year and Date
      2015-08-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Tryptophan enhances the reproductive organs-specific expression level of an amino acid transporter homolog, Dr-SLC38A9 to promote sexual induction of the planarian Dugesia ryukyuensis2015

    • Author(s)
      Maezawa T, Ishikawa M, Nagamatsu G and Kobayashi K
    • Organizer
      International Symposium of Flatworm Biology
    • Place of Presentation
      Oxford, United Kingdom
    • Year and Date
      2015-08-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラナリア生殖器官に特異的に発現するアミノ酸トランスポーターホモログ遺伝子の機能解析2015

    • Author(s)
      前澤孝信、石川正樹、小林一也
    • Organizer
      中国四国地区生物系三学会合同大会
    • Place of Presentation
      愛媛
    • Year and Date
      2015-05-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] Tryptophan enhances the reproductive organs-specific expression level of an amino acid transporter homolog, Dr-slc38A9 in the sexual worms of Dugesia ryukyuensis2015

    • Author(s)
      Masaki Ishikawa, Takanobu Maezawa and Kazuya Kobayashi
    • Organizer
      1st Asian PlanNet Meeting
    • Place of Presentation
      香港
    • Year and Date
      2015-05-07 – 2015-05-09
    • Related Report
      2014 Research-status Report
  • [Presentation] プラナリア生殖器官特異的に発現するアミノ酸輸送体ホモログの解析2014

    • Author(s)
      石川正樹, 前澤孝信, 小林一也
    • Organizer
      日本動物学会第84回大会
    • Place of Presentation
      仙台
    • Year and Date
      2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] D-AMINO ACID OXIDASE IS INVOLVED IN THE OVARIAN DEVELOPMENT OF ASEXUAL PLANARIAN WORMS2014

    • Author(s)
      Hiroyuki TANAKA, Takanobu MAEZAWA, Yoshihiro NISHIMURA, Tetsuo ISHIDA, Kazuya KOBAYASHI, Yasutoshi AGATA
    • Organizer
      The 2nd International Conference of D-Amino Acid Research
    • Place of Presentation
      宇都宮
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] プラナリア生殖器官に特異的に発現するアミノ酸トランスポーターホモログ遺伝子の機能解析2014

    • Author(s)
      前澤孝信、石川正樹、小林一也
    • Organizer
      日本動物学会 平成26年度中国•四国支部大会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2014-05-11
    • Related Report
      2014 Research-status Report
  • [Presentation] Switching from asexual to sexual reproduction in the planarian Dugesia ryukyuensis:The role of D-amino acid oxidase

    • Author(s)
      Takanobu Maezawa, Hiroyuki Tanaka and Kazuya Kobayashi
    • Organizer
      第46回日本生発生生物学会大会
    • Place of Presentation
      松江
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] プラナリア生殖器官に特異的に発現するアミノ酸トランスポーターホモログ遺伝子の機能解析

    • Author(s)
      前澤孝信、石川正樹、小林一也
    • Organizer
      日本動物学会中国四国地区支部 第66回大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Research-status Report
  • [Book] Reproductive and Developmental Strategies2016

    • Author(s)
      Maezawa T, Ishikawa M, Okamoto H and Kobayashi K
    • Publisher
      Springer Japan
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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