Molecular mechanisms regulating conversion of pluripotential stem cells into Germ cells
Project/Area Number |
25650104
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Morphology/Structure
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Research Institution | Tohoku University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI KAZUYA 弘前大学, 農学生命科学部, 准教授 (50360110)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 多能性幹細胞 / 生殖細胞 / RNA干渉法 / プラナリア / ニワトリ / Max / Brg1 |
Outline of Final Research Achievements |
We previously found that knock-down of transcription factors, Max and Brg1 in mouse embryonic stem cells (ESCs) by RNA interference (RNAi) resulted in global induction of germ cell-specific genes. In this study, we attempted to examine whether Max and Brg1 also play a similar role on pluripotential stem cells in diverse organisms such as chick and planaria. We found that Max- or Brg1-knockdown (KD) in mature planaria containing pluripotential neoblast cells resulted in up-regulation of some germ cell-specific genes. We also found increased expression of some germ cell-specific genes by Max-KD in pluripotential blastoderm cells derived from early chick embryos. The results indicate that Max and Brg1 are involved in repression of germ cell-specific genes in planaria and chick, but they repress only a part of germ cell-specific genes. Therefore functions of Max and Brg1 may be somewhat different among mouse ESCs, planaria and chick pluripotential stem cells.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] The protein phosphatase 6 catalytic subunit (Ppp6c) is indispensable for proper post-implantation embryogenesis.2016
Author(s)
Ogoh, H., Tanuma, N., Matsui, Y., Hayakawa, N., Inagaki, A., Sumiyoshi, M., Momoi, Y., Kishimoto, A., Suzuki, M., Sasaki, N., Ohuchi, T., Nomura, M., Teruya, Y., Yasuda, K., Watanabe, T., Shima, H.
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Journal Title
Mechanisms of Development
Volume: 139
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Max as a biological blockade that restricts meiotic process in ESCs.2016
Author(s)
Suzuki A, Hirasaki M, Hishida T, Okamura D, Wu J, Ueda A, Nishimoto M, Nakachi Y, Mizuno Y, Okazaki Y, Matsui Y, *Izpisua Belmonte JC, Okuda A.
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Journal Title
Nat Commun
Volume: 7
Issue: 1
Pages: 11056-11056
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Sex Specification and Heterogeneity of Primordial Germ Cells in Mice.2015
Author(s)
Sakashita, A., Kawabata, Y., Jincho, Y., Tajima, S., Kumamoto, S., Kobayashi, H., Matsui, Y. and Kono, T.
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Journal Title
PLoS ONE
Volume: 10
Issue: 12
Pages: 1-21
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The ADP-ribosylation factor 1 gene is indispensable for mouse embryonic development after implantation.2014
Author(s)
Hayakawa, N., Ogoh, H., Sumiyoshi, M., Matsui, Y., Nishikawa, S., Miyamoto, K., Maede, Y., Kiyonari, H., Suzuki, M. and Watanabe, T.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 453
Issue: 4
Pages: 748-753
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] On the fate of primordial germ cells injected into early mouse embryos.2013
Author(s)
Leitch, H.G., Okamura, D., Durcova-Hills, G., Stewart, C.L., Gardner, R.L., Matsui, Y., Papaioannou, V.E.
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Journal Title
Developmental Biology
Volume: 385
Issue: 2
Pages: 155-159
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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