Does bacterial membrane vesicles isolated from environmental samples possess a role of carrierrs for bacterial signals

• Project/Area Number: 25650105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: University of Tsukuba
• Principal Investigator: NOMURA Nobuhiko 筑波大学, 生命環境系, 教授 (60292520)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: メンブランベシクル / 細菌 / 細菌シグナル / アシル化ホモセリンラクトンのシグナル

Outline of Final Research Achievements

Almost bacteria naturally secrete sphere shaped vesicles that are approximately 50 to 250 nm. These vesicles are mainly composed of cellular membrane and hence called membrane vesicles (MVs). It has been reported about MV, but they were about MV of model bacteria. It doesn't have been reported about MV from environmental samples. Here, we investigated that bacterial membrane vesicles isolated from environmental samples include a role as a carrier of bacterial signals.

Research Products

• [Journal Article] A novel approach for purification and selective capture of membrane vesicles of the periodontopathic bacterium, Porphyromonas gingivalis: membrane vesicles bind to magnetic beads coated with epoxy groups in a noncovalent, species-specific manner. (2014)
  • Author(s): Nakao R, Kikushima K, Higuchi H, Obana N, Nomura N, Bai D, Ohnishi M, Senpuku H.
  • Journal Title: PLoS One. Volume: 9 Issue: 5 Pages: 1-10
  • DOI: 10.1371/journal.pone.0095137
  • Peer Reviewed / Open Access
• [Journal Article] Membrane vesicle formation is associated with pyocin production under denitrifying conditions in Pseudomonas aeruginosa PAO1. (2014)
  • Author(s): Toyofuku M, Zhou S, Sawada I, Takaya N, Uchiyama H, Nomura N
  • Journal Title: Environ Microbiol. Volume: 印刷中 Issue: 9 Pages: 2927-2938
  • DOI: 10.1111/1462-2920.12260
  • Peer Reviewed
• [Presentation] メンブランベシクルから見えてくる新たな微生物間コミュニケーション、バイオフィルム像 (2014)
  • Author(s): 豊福雅典、Leo Eberl、野村暢彦
  • Organizer: 生物工学会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌）
  • Year and Date: 2014-09-09 – 2014-09-10
  • Invited
• [Presentation] 微生物の城づくり ～微生物も会話し集団生活している～
  • Author(s): 野村暢彦
  • Organizer: 第2回日本微生物学連盟フォーラム『微生物―知られざるミクロのエンジニア』
  • Place of Presentation: 東京大学駒場キャンパス
  • Invited
• [Presentation] 微生物間コミュニケーションを利用したバイオフィルム制御技術の開発
  • Author(s): 野村暢彦
  • Organizer: 藪田セミナー
  • Place of Presentation: アクトシティ浜松コングレスセンター
  • Invited
• [Book] Biodegradative Bacteria (2014)
  • Author(s): Masanori Toyofuku, Nobuhiko Nomura
  • Total Pages: 17
  • Publisher: Springer