| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
The characteristics of columnar cells of the peritoneal epithelium in slender cords were examined in newt, Cynops pyrrhogaster. The columnar cells were not positive for VASA or SGSA-1 antibodies, which are recognized germ cells containing gonial germ cells specifically. These cells did not show the localization of nuage, which is reported as germ cell specific electron-dense structure. After the treatment of estrogen benzoates, several columnar cells showed the VASA-positive. Furthermore, nuage structure was seen in these VASA-positive cells by electron microscopy. These results suggest the possibility that the columnar cell of the peritoneal epithelium in slender cords in the newt can trans-differentiate into gonial germ cell like cells after estrogen treatment.
|
