| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
In vertebrates, boric acid is a vital micronutrient that is required for embryogenesis and cell growth. High [boric acid] is toxic to animals, however, little is known about the whole-body boric acid homeostasis. We found that marine teleosts, e.g., Takifugu obscurus and Takifugu rubripes, excrete and concentrate boric acid in the urine to eliminate excess boric acid, and identified channels for borate (Slc4a11) and boric acid (AQP3, 7, 8, 9a, 9b) in pufferfish. To clarify the presence of similar systems in mammals, the functions of mammalian Slc4a11 and AQPs were analyzed using ion-selective microelectrodes. Human AQP3, 7, 8, 9, 10 showed boric acid and water channel activities. However, human and mouse Slc4a11 showed no significant borate-transport activity. These results indicate that mammalian boric acid homeostasis is mainly maintained by AQP family whereas that of marine teleosts is regulated by Slc4a11 and AQPs.
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