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Elucidation of molecular analysis for chloroplast genome translocation using versatile model-plant Marchantia

Research Project

Project/Area Number 25650126
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Research InstitutionInstitute of Physical and Chemical Research (2014-2015)
Kyoto University (2013)

Principal Investigator

Ueda Minoru  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (30632541)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsオルガネラゲノム収奪 / 進化 / 葉緑体 / ゼニゴケ / 葉緑体ゲノム収奪 / 葉緑体形質転換
Outline of Final Research Achievements

Having efficient chloroplast genome transformation and genetic screening system established in Marcantia, measurement of genome translocation rate from chloroplast genome to the nucleus has been done. Analysis of measurement of genome translocation rate revealed that the translocation rate is quite low in Marchantia. Because Marchantia suppresses the translocation, genetic screening using T-DNA insertion lines has been done to find a mutant in which the translocation occurs. As a result, one mutant has been isolated and another allele for the gene was prepared by gene targeting. Now, I am not able to conclude because NGS analysis have not detected any novel chloroplast genome insertion in the nucleus in mutants generated by gene targeting. I am still analyzing while improving method.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2016 2014 2013 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha.2016

    • Author(s)
      Boehm CR, Ueda M, Nishimura Y, Shikanai T, Haseloff J
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 2 Pages: 291-299

    • DOI

      10.1093/pcp/pcv160

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] chlB requirement for chlorophyll biosynthesis under short photoperiod in Marchantia polymorpha L.2014

    • Author(s)
      Ueda M, Tanaka A, Sugimoto K, Shikanai T and Nishimura Y
    • Journal Title

      Genome Biol Evol.

      Volume: 6(3) Issue: 3 Pages: 620-628

    • DOI

      10.1093/gbe/evu045

    • NAID

      120005458285

    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Subfunctionalization of sigma factors during the evolution of land plants based on mutant analysis of liverwort (Marchantia polymorpha L.) MpSIG1.2013

    • Author(s)
      Ueda, M., Takami, T., Peng, L., Ishizaki, K., Kohchi, T., Shikanai, T. and Nishimura, Y.
    • Journal Title

      Genome Biology and Evolution

      Volume: 5 Issue: 10 Pages: 1836-1848

    • DOI

      10.1093/gbe/evt137

    • NAID

      120005373074

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Identification of a gene which is in the intermediate stage of gene loss from chloroplast genome in Marchantia polymorpha

    • Author(s)
      Ueda, M., Tanaka, A., Shikanai, T., Nishimura, Y.、
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Research-status Report
  • [Presentation] Marchantia plastid (chloroplast) transformation for the study of endosymbiosis

    • Author(s)
      Ueda, M., Tanaka, A., Shikanai, T., Nishimura, Y.
    • Organizer
      International Colloquium on Endocytobiology and Symbiosis
    • Place of Presentation
      Dalhousie University, Canada
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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