Examination of the codon-acquisition hypothesis in the early evolution of life
| Project/Area Number |
25650127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | RNA複製酵素 / コドン / アミノ酸 / RNA / 進化 / 実験進化 / アミノ酸獲得仮説 |
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| Outline of Final Research Achievements |
Present-day proteins consist of mainly 20 amino acids, while ancient proteins are considered to consist of fewer amino acids. In this study, I attempted to examine this hypothesis through developing an RNA replication enzyme that consists of fewer than 20 amino acids by using an evolvable RNA replication system that we have established. We succeeded to reduce the number of methionine codons in the RNA replicase from 8 to 2, but further reduction abolish the repllication activity. This result demonstrates that the development of an RNA replicase consists of 19 amino acids requires evolution under more different conditions, such as the completely methionine-depleted condition.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment2013
Author(s)
Ichihashi, N., Usui, K., Kazuta, Y., Sunami, T., Matsuura T., Yomo, T
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Journal Title
Nature Communications
Volume: 4
Issue: 1
Pages: 1-7
DOI
NAID
Related Report
Peer Reviewed
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