The role of non-S DNA synthesis on the local mutation rate
| Project/Area Number |
25650130
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Evolutionary biology
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| Research Institution | Kyushu University |
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Principal Investigator |
OHNO Mizuki 九州大学, 医学(系)研究科(研究院), 助教 (70380524)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 分子進化 / 突然変異 / 生殖細胞 / ゲノム変異 / 非S期DNA合成 |
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| Outline of Final Research Achievements |
A major source of spontaneous mutations is DNA replication error; therefore, a mutation rate depends on the frequency of DNA replication. However, local mutation rate varies across the genome. It is well known that the non-S DNA syntheses naturally occur in non-S phase cells in vivo. It is speculated that, in the genomic regions having a tendency of non-S DNA synthesis, mutation rate may be increased by the increased frequency of DNA synthesis. The role of non-S DNA synthesis on the local mutation rate was assessed by analyzing the incorporation of BrdU in the pachytene spermatocytes isolated from the mouse testes. Colocalization of BrdU and SCP3 (a maker for synaptonemal complex) immunofluorescence signals was detected in non-S pachytene spermatocytes, suggesting that non-S DNA synthesis detected here descend from a homologous recombination-dependent DNA synthesis.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] Oxidative DNA damage and its repair system: implications for de novo germline mutations2015
Author(s)
Mizuki Ohno, Noriko Takano, Kunihiko Sakumi, Ryutaro Fukumura, Yuki Iwasaki, Toshimichi Ikemura, Yoichi Gondo, Yusaku Nakabeppu, Yoshimichi Nakatsua, Teruhisa Tsuzuki
Organizer
Genome Integlity, Zing Conference
Place of Presentation
Cairns, Australia
Year and Date
2015-08-01 – 2015-08-05
Related Report
Invited
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[Presentation] 酸化ストレス誘発突然変異と消化管がんの解析2014
Author(s)
大野 みずき, 鷹野典子, 佐々木史子, 李贊, 田口健一, 中別府 雄作, 中津 可道, 續 輝久
Organizer
日本放射線影響学会第57回大会
Place of Presentation
鹿児島
Year and Date
2014-10-01 – 2014-10-03
Related Report
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