| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Numbers of antibiotic-producing strains have been identified from an Actinobacteria, Rhodococcus erythropolis. Each of the strain produces different antibiotic, whereas they are close relatives. The 16S rRNA gene sequence analysis did not classify them because of the high identity. The gyrB sequence analysis for 21 strain was performed to see the phylogenetic relation. In addition, complete genome analysis was done for the 4 of them. The MLST analysis was also tested with rpoB, recA, trpB, atpD and gyrB. These analysis demonstrated that classification by gyrB or rpoB are good match with antibiotic classification. Based on these results, we concluded that the novelty of the product can be judged by the gyrB or rpoB sequence when new candidates are obtained. Construction of database with these information helps to identify novel antibiotics and its producers.
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