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Physiological and genetic characterization of uncultured methanogenic archaea via an ultra-high sensitive fluorescence in situ hybridization technique

Research Project

Project/Area Number 25650138
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biodiversity/Systematics
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

IMACHI Hiroyuki  国立研究開発法人海洋研究開発機構, 深海・地殻内生物圏研究分野, 主任研究員 (20361933)

Co-Investigator(Renkei-kenkyūsha) KUBOTA Kengo  東北大学, 工学研究科, 准教授 (80455807)
Research Collaborator SAITO Yayoi  
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsFISH法 / メタン菌 / mcrA遺伝子 / 生態
Outline of Final Research Achievements

The investigation of uncultured methanogens is a significant challenge in comprehensive understanding global carbon cycle. In this study, we aimed to gain physiological and genetic information of taxonomically new methanogens by obtaining genomic sequences and isolate. For this purpose, we first performed mcrA and 16S rRNA gene-based clone analyses on methanogenic sludges to explore taxonomically novel methanogens. Through the analyses, we found a novel methanogen group, which may represent the eighth order of methanogen. To obtain genetic information of the methanogen, we tried to do specific sorting of the methanogen cells using a combination of an ultra-high sensitive fluorescence in situ hybridization (FISH) technique (i.e., two-pass tyramide signal amplification FISH with polynucleotide probes) and fluorescence-activated cell sorting. However, unfortunately, the targeted methanogen cells could not be detected by the FISH technique even after several cell wall treatments.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Presence of a novel methanogenic archaeal lineage in anaerobic digesters inferred from mcrA and 16S rRNA gene phylogenetic analyses2015

    • Author(s)
      Saito Y, Aoki M, Hatamoto M, Yamaguchi T, Takai K and Imachi H
    • Journal Title

      Journal of Water and Environmental Technology

      Volume: 13 Pages: 279-289

    • NAID

      130005076093

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

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Published: 2014-07-25   Modified: 2019-07-29  

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