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Bacterial autecology in soil environments by using proteomics

Research Project

Project/Area Number 25650139
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionHokkaido University

Principal Investigator

KASAHARA Yasuhiro  北海道大学, 低温科学研究所, 准教授 (20273849)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsメタプロテオーム / 土壌細菌 / 個生態学
Outline of Final Research Achievements

To detect soil-specific expressed genes, comparative proteome analysis of the global behavior of Pseudomonas putida F1 between three soils and liquid batch culture was performed. (1) We studied direct and indirect protein extraction methods by comparing the number of the identified proteins and their functional and taxonomical distributions. We demonstrated the advantages and disadvantages of the two extraction methods that can be applied to complex natural soils. (2) Two genes and 1 operon consisted of 5 genes as a soil-specific expressed gene were identified. From expression factor analysis, one of the genes was expressed by NO. Expression of the operon was found to be the lack of certain soil components. It became possible to know the microenvironment surrounding the bacteria in this study.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] An essential enzyme for phospholipid synthesis has a crucial role in Bacillus subtilis cell division2014

    • Author(s)
      Hiraku Takada, Sanae Fukushima-Tanaka, Masato Morita, Yasuhiro Kasahara, Satoru Watanabe, Taku Chibazakura, and Hirofumi Yoshikawa
    • Journal Title

      Mol Microbiol

      Volume: 91(2) Issue: 2 Pages: 242-255

    • DOI

      10.1111/mmi.12457

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metaproteomic Identification of Diazotrophic Methanotrophs, and their Tissue Localization in Field-grown Rice Roots2014

    • Author(s)
      Bao, Z., T. Okubo, K. Kubota, Y. Kasahara, H. Tsurumaru, M. Anda, S. Ikeda, K. Minamisawa
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 80 Issue: 16 Pages: 5043-5052

    • DOI

      10.1128/aem.00969-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gene expression profiling of Pseudomonas putida F1 after exposure to aromatic hydrocarbon in soil by using proteome analysis2013

    • Author(s)
      H. Morimoto, M. Kuwano and Y. Kasahara
    • Journal Title

      Archives of Microbiology

      Volume: 195 Issue: 12 Pages: 805-813

    • DOI

      10.1007/s00203-013-0932-4

    • NAID

      120005522323

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 水稲根における窒素固定メタン酸化細菌のメタプロテオーム同定と組織局在性2014

    • Author(s)
      包智華、大久保卓、久保田健吾、笠原康裕、鶴丸博人、按田瑞恵、池田成志、南澤 究
    • Organizer
      日本土壌肥料学会2014年度大会
    • Place of Presentation
      東京農工大学(東京都府中市)
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 根粒菌シグマ因子RpoH1の制御を受ける機能未知遺伝子の鉄硫黄タンパク質生合成へ の関与2014

    • Author(s)
      佐々木祥平、門屋亨介、笠原康裕、南澤究、三井久幸
    • Organizer
      第8回日本ゲノム微生物学会
    • Place of Presentation
      東京農業大学(東京都世田谷区)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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