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Development of a quick method to construct linkage maps for genome-scan analysis in non-model plant species

Research Project

Project/Area Number 25650140
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ecology/Environment
Research InstitutionTohoku University

Principal Investigator

SUYAMA Yoshihisa  東北大学, (連合)農学研究科(研究院), 准教授 (60282315)

Research Collaborator MATSUKI Yu  
NAKAZAWA Fumio  
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords連鎖解析 / 次世代シーケンサー / 花粉DNA / 花粉
Outline of Final Research Achievements

We developed a quick method to construct linkage maps with pollen DNA using next-generation sequencer (NGS). As one of the applicable techniques in this study, we developed a PCR-based procedure to construct reduced representation libraries, representing de novo single nucleotide polymorphism (SNP) discovery, and its genotyping using NGS. We then demonstrated the applicability of the method in pollen DNA analysis. Mendelian 1:1 segregation in haploid pollen grains from one individual plant was demonstrated for 226 SNP markers discovered by the method. We concluded that this approach is applicable to construct linkage maps in non-model plant species.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015 2014

All Presentation (3 results) (of which Invited: 2 results)

  • [Presentation] Development of a novel method for conservation genetics with next-generation sequencing: Multiplexed ISSR (inter-simple sequence repeat) Genotyping by sequencing (MIG-seq)2015

    • Author(s)
      Yoshihisa Suyama, Yu Mastuki
    • Organizer
      Contribution of Genetics to Plant Conservation: International Seminar in Rio de Janeiro Botanical Garden
    • Place of Presentation
      Rio de Janeiro Botanical Garden(リオデジャネイロ、ブラジル)
    • Year and Date
      2015-02-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Development of a novel method for conservation genetics with next-generation sequencing: Multiplexed ISSR (inter-simple sequence repeat) Genotyping by sequencing (MIG-seq)2015

    • Author(s)
      Yoshihisa Suyama, Yu Mastuki
    • Organizer
      Contribution of Genetics to Plant Conservation: Brazil-Japan International Workshop 2015
    • Place of Presentation
      University of Sao Paulo(サンパウロ、ブラジル)
    • Year and Date
      2015-02-02 – 2015-02-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 次世代DNAシーケンシングによる森林分子生態学的研究2014

    • Author(s)
      陶山佳久
    • Organizer
      第125回日本森林学会大会
    • Place of Presentation
      大宮ソニックシティー
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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