A pilot study for elucidating molecular mechanisms regulating sexual dimorphism and diversity of human and non-human primates

• Project/Area Number: 25650154
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical anthropology
• Research Institution: Kitasato University
• Principal Investigator: OOTA Hiroki 北里大学, 医学部, 准教授 (40401228)
• Co-Investigator(Kenkyū-buntansha): FURUICHI Takeshi 京都大学, 霊長類研究所, 教授 (20212194)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 性的二型 / ニホンザル / 排卵 / 発情期 / エストロゲン / プロゲステロン / 遺伝子発現 / RNAseq / フィールド試料 / 性皮腫張 / 性周期 / 性ホルモン / ヒト / PER2遺伝子

Outline of Final Research Achievements

[Purpose] In this study, we try to provide a list of the genes that change the expression levels in phase with oscillation of sex hormones. [Materials&Methods] Blood samples were collected from two Japanese macaques with spontaneous ovulation. The blood concentrations of estradiol and progesterone were measured with time. We extracted RNAs from the blood at the peaks of the hormone concentrations just before and after ovulation, and conducted RNAseq for the four samples. [Progress] We are going to analyze the raw data of RNAseq.

Research Products

• [Journal Article] Natural allelic variations of xenobiotic-metabolizing enzymes affect sexual dimorphism in Oryzias latipes (2014)
  • Author(s): Katsumura T., Oda S., Nakagome S., Hanihara T., Kataoka H., Mitani H., Kawamura S., Oota H.
  • Journal Title: Proceedings of The Royal Society B Volume: 281 Issue: 1797 Pages: 1-8
  • DOI: 10.1098/rspb.2014.2259
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Color vision variation as evidenced by hybrid L/M opsin genes in wild populations of trichromatic Alouatta New World monkeys. (2014)
  • Author(s): Matsushita, Y., Oota, H., Welker, B. J., Pavelka, M. S. and Kawamura, S.
  • Journal Title: International Journal of Primatology Volume: 35 Issue: 1 Pages: 71-87
  • DOI: 10.1007/s10764-013-9705-9
  • Peer Reviewed / Open Access
• [Journal Article] Generation of Monoclonal antibodies to Galß1-4Gal epitopes: a key tool in studies of species-specific glycans expressed in fish, amphibians, and birds. (2013)
  • Author(s): Suzuki N,Nawa D,Tateno H,Yasuda T,Oda S,Mitani H,Nishimaki T, Katsumura T,Oota H,Hanihara T,Oga A,Hirabayashi J,Yamamoto K.
  • Journal Title: Glycobiology Volume: 23 Issue: 1 Pages: 91-105
  • DOI: 10.1093/glycob/cws129
  • Peer Reviewed
• [Presentation] 薬物代謝酵素の遺伝的多型がメダカの性的二型に与える影響とその進化的考察 (2014)
  • Author(s): 勝村啓史、尾田正二、三谷啓志、河村正二、太田博樹
  • Organizer: 日本進化学会第16回大阪大会
  • Place of Presentation: 高槻現代劇場（大阪府高槻市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] メダカ体内受精卵の全身組織切片からの組織学的解析ー受精卵の三次元再構築を中心にー (2014)
  • Author(s): 西槙俊之、木森義隆、勝村啓史、尾田正二、埴原恒彦、太田博樹
  • Organizer: 第119回日本解剖学会全国学術集会
  • Place of Presentation: 栃木
• [Presentation] Naturally occurring variation of CYP xenobiotic enzyme alleles in medaka sheds a new light on sexual dimorphism variation among human populations (2013)
  • Author(s): T. Katsumura, S. Oda, H. Mitani, S. Kawamura, H. Oota
  • Organizer: 19th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: Sendai, Japan
• [Presentation] Reconstructing 3D image based on tissue sections of internally fertilized egg in Oryzias latipes (2013)
  • Author(s): T. Nishimaki, Y. Kimori, T. Katsumura, S. Oda, T. Hanihara, H. Oota
  • Organizer: 19th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: Sendai, Japan
• [Presentation] Collaborative efforts for the establishment of human disease model using Medaka Presentation (2013)
  • Author(s): K. Naruse, Y. Taniguchi, S. Kuninaka, S. Oda, M. Shinya, A. Oga, H. Oota, Y. Kamei
  • Organizer: 1st Zebrafish for Personalized/Precision Medicine Conference
  • Place of Presentation: Toronto, Canada
• [Presentation] 動きと組織切片像の数値化によるメダカの全部の定量化
  • Author(s): 尾田正二、浅香智美、保田隆子、三谷啓志、谷口善仁、太田博樹、勝村啓史、西槙俊之、埴原恒彦、小賀厚徳、木森義隆
  • Organizer: メダカを使った解析パイプライン～ヒト疾患モデルとしてのメダカ：コンディショナルKOなどを使った多面的解析系の確立～
  • Place of Presentation: 岡崎コンファレンスセンター、愛知