| Project/Area Number |
25660001
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Tohoku University |
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Principal Investigator |
TORIYAMA Kinya 東北大学, (連合)農学研究科(研究院), 教授 (20183882)
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| Co-Investigator(Renkei-kenkyūsha) |
KAZAMA Tomohiko 東北大学, 大学院農学研究科, 助教 (30431464)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物 |
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| Outline of Final Research Achievements |
To establish transformation system of rice mitochondria, we aimed to develop a mitochondrial artificial chromosome. We employed a utilization of plasmid-like circle DNA, B1 plasmid, which is stably present in mitochondria of BT-type and LD-type cytoplasmic male sterile rice. We carried out cloning of LD-type B1 plasmid and inserted our originally designed GFP expression cassette. Finally we constructed a putative mitochondrial artificial chromosome containing GFP cassette using an E. coli-based mini-circle vector technology to test whether it work as an artificial chromosome in rice mitochondria.
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