An approach toward developing mitochondrial artificial chromosome in rice
| Project/Area Number |
25660001
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Tohoku University |
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Principal Investigator |
TORIYAMA Kinya 東北大学, (連合)農学研究科(研究院), 教授 (20183882)
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| Co-Investigator(Renkei-kenkyūsha) |
KAZAMA Tomohiko 東北大学, 大学院農学研究科, 助教 (30431464)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物 |
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| Outline of Final Research Achievements |
To establish transformation system of rice mitochondria, we aimed to develop a mitochondrial artificial chromosome. We employed a utilization of plasmid-like circle DNA, B1 plasmid, which is stably present in mitochondria of BT-type and LD-type cytoplasmic male sterile rice. We carried out cloning of LD-type B1 plasmid and inserted our originally designed GFP expression cassette. Finally we constructed a putative mitochondrial artificial chromosome containing GFP cassette using an E. coli-based mini-circle vector technology to test whether it work as an artificial chromosome in rice mitochondria.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] Comparative study on cytoplasmic male sterility-associated genes, orf79, orf113, orf352, derived from Oryza rufipogon.2013
Author(s)
Toriyama, K., Igarashi, K., Okazaki, M., Motomura, K., Kazama, T.
Organizer
7th International Rice Genetics Symposium
Place of Presentation
Manila, Philippines
Related Report
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