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Development of high efficient and expression transformation system in plants

Research Project

Project/Area Number 25660004
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionNagoya University

Principal Investigator

SATO Yutaka  名古屋大学, 生命農学研究科, 准教授 (40345872)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsイネ / RNAサイレンシング / RNAサイレンシング
Outline of Final Research Achievements

RNA silencing is known as a defense system against invading exogenous genes such as transposons or RNA viruses. RNA silencing is triggered by the production of double stranded RNA derived from invaders and this results in inactivation or degradation of invading exogenous genes. In case of plant transformation, the double stranded RNAs are often produced from transgenes by means of transcription read through or transgene array. These also can start host's RNA silencing and result in low efficient transformation or low expression of transgene. In this study, I aimed to develop a novel plant transformation system to obtain high expression and high efficient transformants.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] Rice SNF2 family helicase ENL1 is essential for syncytial endosperm development.2015

    • Author(s)
      Tomomi Hara, Hirokazu Katoh, Daisuke Ogawa, Yasuaki Kagawa, Yutaka Sato, Hidemi Kitano, Yasuo Nagato, Ryo Ishikawa, Akemi Ono, Tetsu Kinoshita, Shin Takeda, Tsukaho Hattori
    • Journal Title

      The Plant Journal

      Volume: 81 Issue: 1 Pages: 1-12

    • DOI

      10.1111/tpj.12705

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of the rice microRNA <i>miR820</i> is associated with epigenetic modifications at its own locus2013

    • Author(s)
      Misuzu Nosaka, Akemi Ono, Aiko Ishiwata, Sae Shimizu-Sato, Kiyoe Ishimoto, Yusaku Noda and Yutaka Sato
    • Journal Title

      Genes & Genetic Systems

      Volume: 88 Issue: 2 Pages: 105-112

    • DOI

      10.1266/ggs.88.105

    • NAID

      10031183176

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The copy number of rice CACTA DNA transposon carrying MIR820 does not correlate with MIR820 expression.2013

    • Author(s)
      Misuzu Nosaka, Aiko Ishiwata, Sae-Shimizu Sato, Akemi Ono, Kiyoe Ishimoto, Yusaku Noda, Yutaka Sato
    • Journal Title

      Plant Signal. Behav.

      Volume: 8 Issue: 8 Pages: e25169-e25169

    • DOI

      10.4161/psb.25169

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 外来遺伝子防御システムを利用した導入遺伝子高発現組換えイネの作成2014

    • Author(s)
      小澤美沙、佐藤豊
    • Organizer
      第126回 日本育種学会講演会
    • Place of Presentation
      南九州大学
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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