| Project/Area Number |
25660008
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 有用物質生産 / 葉緑体工学 / 遺伝子発現制御 / 光 / 転写 |
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| Outline of Final Research Achievements |
Costs for production of therapeutic proteins in animal cells decline down to 1/100 when they are made in plants. However, such exogenous proteins frequently interfere with the growth of plants. Therefore, "light-switch" for turning on the expression of foreign genes is needed after plants have grown. “Drought-switch” was also tried to develop. The selectable suppression of transcription of psaA, Arabidopsis chloroplast genome-encoded gene for photosystem (PS) I reaction center protein, was applied to establishment of "light-switch". The optimal light wavelength was determined at 700 nm. Reporter genes, CBRluc for red color luminescence and CBG68luc for green one as provided as Chroma-Glo Luciferase (Promega) were employed. An evaluation protocol on the basis of transient expression was established. A system for turning on the gene expression by light exposure was designed with RNAi, as the psaA regulation was negative.
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