| Project/Area Number |
25660019
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUZUKI TAKASHI 北海道大学, (連合)農学研究科(研究院), 准教授 (30196836)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMURA Hanako 北海道大学, 大学院農学研究院, 助教 (20507230)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | アスパラガス / ウイルスフリー化 / アスコルビン酸 / 凍結処理 / 園芸学 |
|---|
| Outline of Final Research Achievements |
Asparagus virus 1 (AV-1) and Asparagus virus 2 (AV-2) have been considered as major pathogenic viruses that cause the reduction of productivity of asparagus. To prepare the virus-free asparagus in vitro culture for field production, we here tried to establish a new approach for virus elimination from asparagus by using ascorbic acid treatment and slow-freezing cryotherapy. Virus elimination was observed in seedlings treated by either ascorbic acid or slow-freezing, but there were differences in elimination rate among asparagus cultivars. In addition, enhanced growth of asparagus seedling after slow-freezing treatment was observed in some tested plants. AV-2 was more difficult to be eliminated from seedlings than AV-1, probably due to systemic infection by AV-2 in asparagus. Additional immunohistochemical analysis confirmed that AV-2 was localized in the leaf primordia and vascular tissue around shoot meristem of asparagus seedlings.
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