| Project/Area Number |
25660023
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | Shizuoka University |
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Principal Investigator |
Kato Masaya 静岡大学, 農学部, 教授 (10432197)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | β－シトラウリン / カロテノイド / カンキツ / CCD4 / アポカロテノイド |
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| Outline of Final Research Achievements |
β-Citraurin, a C30 apocarotenoid, is a color-imparting pigment responsible for the reddish color of citrus fruits. In the present study, we found that CitCCD4 was involved in the synthesis of β-citraurin, using two citrus varieties of Satsuma mandarin, ‘Yamashitabeni-wase’, which accumulates β-citraurin predominantly, and ‘Miyagawa-wase’, which does not accumulate β-citraurin. The expression of CitCCD4 increased significantly in the flavedo of ‘Yamashitabeni-wase’ during fruit maturation. In addition, function analysis showed that the cleavage of β-cryptoxanthin and zeaxanthin by CitCCD4 enzyme led to the formation of β-citraurin. The results suggested that CitCCD4 was a key gene regulating the biosynthesis of β-citraurin.
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