Analysis of self-incompatibility-related genes of citrus uising single pollen culture system.
| Project/Area Number |
25660029
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticultural science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 自家不和合性 / カンキツ / 成熟花粉 / 配偶体型 / 花粉培養 / プログラム細胞死 / 花柱タンパク質 / SOD / ROS / 生殖生理 / アポトーシス / プロテオーム / マイクロマニピュレーター |
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| Outline of Final Research Achievements |
Self-incompatibility (SI) is an important mechanism in higher plants that promotes outcrossing and prevents self-fertilization. In this study, We analyzed the protein changes in citrus self-incompatible cultivar, ‘Banpeiyu’ and ‘Hyuganatsu’ , pollen tubes with the C- and SI-like treatments by nano-LC/MS; 14 and 27 proteins were identified in C- and SI-like treatments, respectively. We picked up some candidate genes that were particularly prevalent in SI-like treatment and analyzed their expression level changes during C- and SI-like treatments in citrus pollen tubes. The expression levels of copper-zinc superoxide dismutase (Cu/Zn SOD), manganese SOD (Mn SOD), catalase (CAT), and cysteine protease (CYP) increased after SI-like treatment. Since ROS has been proposed as a key inducer of program cell death (PCD), it is possible that the ROS cascade reaction, which was induced by SI-like treatment in citrus pollen tubes, is a trigger for the PCD process.
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Report
(3 results)
Research Products
(5 results)
