| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
3-deoxyanthocyanins are rare plant anthocyanins found in a limited plant species. We attempted to elucidate the 3-deoxyanthocyanin biosynthetic pathway in Sinningia cardinalis for genetic engineering of flower color using these rare pigments. To this end, the flowers of S. cardinalis were subjected to RNA-seq analysis and expression profiles of the related genes were acquired. Next, we analyzed a white-flowered torenia cultivar (Crown White) and identified three mutations including flavanone 3-hydroxylase (F3H) gene which suppression is prerequisite for accumulation of 3-deoxyanthocyanins. By the transformation of Crown White with related genes of Sinningia and sorghum, some transgenic torenia plants showed cream to light-pink flowers. LC-MS analysis indicated that successful accumulation of 3-deoxyanthocyanin (apigeninidin 5-O-glucoside) in the petals. These results demonstrated that genetic engineering of flower color by 3-deoxyanthocyanin pigments was possible in higher plants.
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