Elucidation of 3-deoxyanthdocyanin biosynthetic pathway and genetic engineering of novel flower color
| Project/Area Number |
25660030
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Horticultural science
|
|---|
| Research Institution | Iwate Biotechnology Research Center |
|---|
Principal Investigator |
Nishihara Masahiro 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 研究部長 (20390883)
|
|---|
| Research Collaborator |
YAMADA Eri
FUJITA Kohei
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | フラボノイド / 3-デオキシアントシアニン / 花色 / シンニンギア / 遺伝子工学 / トレニア / タバコ / シニンギア / RNA-seq |
|---|
| Outline of Final Research Achievements |
3-deoxyanthocyanins are rare plant anthocyanins found in a limited plant species. We attempted to elucidate the 3-deoxyanthocyanin biosynthetic pathway in Sinningia cardinalis for genetic engineering of flower color using these rare pigments. To this end, the flowers of S. cardinalis were subjected to RNA-seq analysis and expression profiles of the related genes were acquired. Next, we analyzed a white-flowered torenia cultivar (Crown White) and identified three mutations including flavanone 3-hydroxylase (F3H) gene which suppression is prerequisite for accumulation of 3-deoxyanthocyanins. By the transformation of Crown White with related genes of Sinningia and sorghum, some transgenic torenia plants showed cream to light-pink flowers. LC-MS analysis indicated that successful accumulation of 3-deoxyanthocyanin (apigeninidin 5-O-glucoside) in the petals. These results demonstrated that genetic engineering of flower color by 3-deoxyanthocyanin pigments was possible in higher plants.
|
Report
(4 results)
Research Products
(8 results)
