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Changes in volatiles in vegetables inoculated with ACC deaminase-producing bacteria

Research Project

Project/Area Number 25660033
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Horticultural science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

TANAKA Fukuyo  国立研究開発法人農業・食品産業技術総合研究機構, 中央農業研究センター土壌肥料研究領域, 上級研究員 (50355541)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsACC分解細菌 / エチレン / 揮発性成分 / 植物 / エンドファイト / 香気成分 / ニンジン / ストレス / MPN法 / 品質 / リンゴ / 野菜
Outline of Final Research Achievements

Bacterial ACC deaminase is one of the key tools to ameliorate plant stress by lowering ethylene level in plants. The effects of ACC deaminase-producing bacteria on the volatile profiles in plants have not been examined to date. To address this, we performed metabolic profiling of volatiles in carrots following inoculation of the bacteria producing ACC deaminase
Results:A total of 11 bacterial strains having the activity to cleave ACC were isolated from inner part of vegetables and fruits. Three of them belonged to Pseudomonas could reduce the levels of (E)-2-hexenal and the other volatiles in the leaves of carrot following inoculation of seeds.
Conclusions:The isolated endophytes producing ACC deaminase activity could alter the composition of volatiles in plants, probably through lowering ethylene level in plant.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Changes in volatiles in carrots inoculated with ACC deaminase-producing bacteria isolated from organic crops2016

    • Author(s)
      Hiroaki Matsuoka ,Yoshinari Ohwaki, Junko Terakado-Tonooka, Fukuyo Tanaka
    • Journal Title

      Pant and soil

      Volume: 未定 Issue: 1-2 Pages: 173-186

    • DOI

      10.1007/s11104-015-2769-x

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ACC deaminaseを産生するエンドファイトの接種がニンジンの揮発性成分に及ぼす影響2016

    • Author(s)
      大脇良成,松岡宏明,搭野岡(寺門) 純子,田中福代
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学(岩手県盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] ACC分解細菌の接種がシソの揮発性成分に及ぼす影響2015

    • Author(s)
      田中福代,日本典秀,松岡宏明,搭野岡(寺門) 純子,岡崎圭毅,大脇良成
    • Organizer
      日本土壌肥料学会2015年度京都大会
    • Place of Presentation
      京都大学(京都府京都市)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evaluation of ACC deaminase-producing endophytic bacteria isolated from organic products by mung bean assay2015

    • Author(s)
      Hiroaki Matsuoka ,Yoshinari Ohwaki, Junko Terakado-Tonooka, Fukuyo Tanaka
    • Organizer
      Rhizosphere 4
    • Place of Presentation
      Maastricht Exposition and Congress Centre (オランダ マーストリヒト)
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 作物に生息する有用細菌の選抜2013

    • Author(s)
      田中福代 松岡宏明 塔野岡 (寺門)純子 大脇良成
    • Organizer
      日本土壌肥料学会関東支部会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2013-12-09
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 微生物農薬、並びに植物の虫害抵抗性付与及び品質低下抑制方法2015

    • Inventor(s)
      田中福代 大脇良成 塔野岡純子 日本典秀
    • Industrial Property Rights Holder
      独立行政法人 農業・食品産業技術総合研究機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-029054
    • Filing Date
      2015-02-17
    • Related Report
      2014 Research-status Report

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Published: 2014-07-25   Modified: 2018-12-17  

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