| Project/Area Number |
25660040
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
RYOICHI Sato 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 味受容体 / カイコ / BmGr / 昆虫 / 宿主認識 / 小顋 / レーザーキャプチャーマイクロダイセクション |
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| Outline of Final Research Achievements |
Host plant recognition-concerning taste detection system was attempted to be cleared. First, a member of taste receptors, BmGrs which is expressing in a taste sensing cell of epipharyngeal sensillum in the labrum of Bombyx mori larvae was attempted to be cleared. A neural cell cluster of epipharyngeal sensillum was obtained using glass-capillary and single cell was dissected from it by laser capture microdissection (LCM). RT-PCT suggested that this cell expressed 6 kinds of BmGrs including BmGr10 and they were considered to construct inositol receiving system. Ca2+ indicators, GCaMP co-expressing system and Xenopus oocyte expressing system connected to two-electrode voltage clamp were used to determine the ligand of BmGr10. From these, inositol was demonstrated to be a ligand of BmGr10.
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