• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Reverse toxicological search for new miticides from species specific target sites.

Research Project

Project/Area Number 25660044
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant protection science
Research InstitutionKobe University

Principal Investigator

TAKEDA Makio  神戸大学, (連合)農学研究科(研究院), 教授 (20171647)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
KeywordsGABA受容体 / 殺ダニ剤 / 神経伝達物質 / 塩化物イオンチャンネル / ナミハダニ / RDL受容体 / ディルドリン抵抗性 / フィプロニル / GABA受容体 / RDL受容体 / 有機塩素剤 / ミツバチヘギイタダニ / ヒョウケダニ
Outline of Final Research Achievements

Cloning of GABA receptor from the two spotted spider mite (TSSM), Tetranychus urticae revealed a substitution of a critical amino acid at the second transmembrane damain of a GABA recptor, RDL that is conserved in insects. TSSM GABA receptor has a unique spectrum in action of mitocids and non-competitive GABAR agonists from ticks and insects. We made a chimera construct to narrow down the critical structure to establish effective screening for specific miticides, employing two electrode voltage clamp method. The result however indicated that not only the second trans-membarne domain but multiple other sites are sensitive to these compounds, especially Cyc-loop region, which complicated the strategy of effective screen system for specific miticidal compounds contrary to our original expectation.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013

All Journal Article (11 results) (of which Peer Reviewed: 7 results)

  • [Journal Article] An LED-based UV-B irradiation system for tiny organisms: System description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa2014

    • Author(s)
      Suzuki, T., Y. Yoshioka, O. Tsarsitalidou, V. Ntalia, S. Ohno, K. Ohyama, Y. Kitashima, T. Gotoh, M. Takeda and D.S. Koveos
    • Journal Title

      Journal of Insect Physiology

      Volume: 62 Pages: 1-10

    • DOI

      10.1016/j.jinsphys.2014.01.005

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] N-acetyltransferase (nat) is a critical conjunct of photoperiodism between the circadian system and endocrine axis in Antheraea pernyi2014

    • Author(s)
      Ahmed A. M. M., Q.-S. Wang, J. Bembenek, N. Ichihara, S. Hiragaki, T. Suzuki and M. Takeda
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 3 Pages: e92680-e92680

    • DOI

      10.1371/journal.pone.0092680

    • NAID

      120005465538

    • Related Report
      2013 Research-status Report
  • [Journal Article] Characterization of rab-interacting lysozomal protein in the brain of Bombyx mori2014

    • Author(s)
      Uno T, Isoyama Y, Sakamoto K, Uno Y, Sakamoto K, Kanamaru K, Yamagata H, Takagi M, Mizoguchi A, Takeda M
    • Journal Title

      Histochem.Cell Biol

      Volume: 141 Issue: 3 Pages: 31-20

    • DOI

      10.1007/s00418-013-1160-3

    • Related Report
      2013 Research-status Report
  • [Journal Article] Cloning of PaAtg8 and roles of autophagy in adaptation to starvation with respect to the fat body and midgut of the Amerian cockroach, Periplaneta americana2014

    • Author(s)
      Moo Soo Park and Makio Takeda
    • Journal Title

      Cell and Tissue Research

      Volume: - Issue: 2 Pages: 405-416

    • DOI

      10.1007/s00441-014-1802-3

    • Related Report
      2013 Research-status Report
  • [Journal Article] The pars intercerebralis affects digestive activities of the American cockroach, Periplaneta americana, via crustacean cardioactive peptide and allatostatin-A.2013

    • Author(s)
      Matsui, T., Sakai, T., Satake, H., Takeda M.
    • Journal Title

      J. Insect Physiol.

      Volume: 59 Issue: 1 Pages: 33-37

    • DOI

      10.1016/j.jinsphys.2012.06.010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Life cycle traits of the giant water bug, Lethocerus deyrollei (Hemiptera: Belostomatidae) in central Japan: possibility of inoculation in extinct areas.2013

    • Author(s)
      Nagaba Y, M Takeda
    • Journal Title

      Environ. Entomol

      Volume: 42 Issue: 2 Pages: 287-295

    • DOI

      10.1603/en12208

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photo‐orientation regulates seasonal habitat selection in the two‐spotted spider mite Tetranychus urticae2013

    • Author(s)
      Takeshi Suzuki, Takeshi Kojima, Makio Takeda, Masayuki Sakuma
    • Journal Title

      Journal of Experimental Biology

      Volume: 216 Pages: 977-983

    • DOI

      10.1242/jeb.079582

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between the expression of Rab family GTPase and neuropeptide hormones in the brain of Bombyx mori. Histochem.2013

    • Author(s)
      Uno T., Sakamoto K, Isoyama Y, Hiragaki S, Uno Y, Kanamaru K, Yamagata H, Takagi M, Mizoguchi A, Takeda M.
    • Journal Title

      Histochem. Cell Biol

      Volume: 139 Issue: 2 Pages: 299-308

    • DOI

      10.1007/s00418-012-1021-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Roles of fat body trophocytes, mycetocytes and urocytes in the American cockroach, Periplaneta americana under starvation conditons: An ultrastructural study2013

    • Author(s)
      Park MS, Park PM , Takeda M.
    • Journal Title

      Arthropod Structure & Development

      Volume: 42 Issue: 4 Pages: 287-295

    • DOI

      10.1016/j.asd.2013.03.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning, characterization, and expression of ultraspiracle gene homolog(RXR/USP) from the hemimetabolous insect Periplaneta american (Dictyoptera, Blattidae) during vitellogenesis2013

    • Author(s)
      Azza M.Elgendy, M Elmogy, M. Takeda
    • Journal Title

      Molecular Biotechnology

      Volume: - Issue: 2 Pages: 126-135

    • DOI

      10.1007/s12033-013-9688-y

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Serotonin receptor B may lock the gate of PTTH release/synthesis in the Chinese silk moth, Antheraea pernyi; a diapause initiation/maintenance mechanism?2013

    • Author(s)
      Wang QS., Ahmed MM. Takeda M.
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 11 Pages: e79381-e79381

    • DOI

      10.1371/journal.pone.0079381

    • NAID

      120005465537

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi